When bred to strains expressing Cre recombinase in various tissues these floxed mice may be useful in studies of insulin resistance, scarring alopecia, pulmonary arterial hypertension, osteoclastogenesis and milk production (inflammatory milk), and vascular nitric oxide production and endothelial cell dysfunction as examples.
Ronald M. Evans, The Salk Inst for Biological Studies
These mice possess loxP sites on either side of exons 1 and 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
When bred to a strain expressing Cre recombinase in adipose tissue (see Stock No. 005069 for example), this mutant mouse strain may be useful in studies of insulin resistance.
When bred to mice carrying Tg(Krt1-15-cre/PGR)22Cot (Stock No. 005249), Cre recombinase expression in the epithelial stem cells results in scarring alopecia.
When bred to mice carrying Tg(Tagln-cre)1Her (Stock No. 017491), Cre recombinase expression in vascular smooth muscle results in pulmonary arterial hypertension (PAH).
When bred to mice carrying Tg(Tek-cre)1Ywa (Stock No. 008863), Cre recombinase expression in the endothelial cells results in alterations in osteoclastogenesis, milk production (inflammatory milk), and hypertension.
When bred to mice carrying Tg(Tek-cre)12Flv (Stock No. 004128), Cre recombinase expression in the endothelial cells results in reduced vascular nitric oxide production and endothelial cell dysfunction.
When bred to mice carrying Tg(Lhb-cre)1Sac (Stock No. 009643), Cre recombinase expression in in pituitary cells that produce luteinizing hormone results in luteinizing hormone, a decrease in follicle-stimulating hormone and fertility defects.
When bred to mice carrying Tg(Cd4-cre)1Cwi (Stock No. 017336), Cre recombinase expression in in CD4 expressing T cells results in increased T-helper 17 cell differentiation.
|Allele Name||targeted mutation 2, Ronald M Evans|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||PPARgammaf; PPARgammafl; Ppargflox; gf|
|Gene Symbol and Name||Pparg, peroxisome proliferator activated receptor gamma|
|Gene Synonym(s)||CIMT1; GLM1; NR1C3; Nr1c3; PPAR-gamma; PPARG1; PPARG2; PPARgamma; PPARgamma2; Ppar-gamma2|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A single loxP site was introduced upstream of exon 1 and a floxed neomycin cassette was inserted downstream of exon 2. The neomycin gene was excised from correctly targeted ES cells by transient transfection with a Cre expression plasmid.|
|Mutations Made By|| |
Yaacov Barak, University of Pittsburgh
When using the PPARγloxP mouse strain in a publication, please cite the originating article(s) and include JAX stock #004584 in your Materials and Methods section.
|Heterozygous for Pparg<tm2Rev>|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in
order to provide the minimum number of animals, animals will ship within 25 weeks.
The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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