When bred to strains expressing Cre recombinase in various tissues these floxed mice may be useful in studies of insulin resistance, scarring alopecia, pulmonary arterial hypertension, osteoclastogenesis and milk production (inflammatory milk), and vascular nitric oxide production and endothelial cell dysfunction as examples.
Ronald M. Evans, The Salk Inst for Biological Studies
These mice possess loxP sites on either side of exons 1 and 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
When bred to a strain expressing Cre recombinase in adipose tissue (see Stock No. 005069 for example), this mutant mouse strain may be useful in studies of insulin resistance.
When bred to mice carrying Tg(Krt1-15-cre/PGR)22Cot
(Stock No. 005249), Cre recombinase expression in the epithelial stem cells results in scarring alopecia.
When bred to mice carrying Tg(Tagln-cre)1Her (Stock No. 017491), Cre recombinase expression in vascular smooth muscle results in pulmonary arterial hypertension (PAH).
When bred to mice carrying Tg(Tek-cre)1Ywa (Stock No. 008863), Cre recombinase expression in the endothelial cells results in alterations in osteoclastogenesis, milk production (inflammatory milk), and hypertension.
When bred to mice carrying Tg(Tek-cre)12Flv (Stock No. 004128), Cre recombinase expression in the endothelial cells results in reduced vascular nitric oxide production and endothelial cell dysfunction.
When bred to mice carrying Tg(Lhb-cre)1Sac (Stock No. 009643), Cre recombinase expression in in pituitary cells that produce luteinizing hormone results in luteinizing hormone, a decrease in follicle-stimulating hormone and fertility defects.
When bred to mice carrying Tg(Cd4-cre)1Cwi (Stock No. 017336), Cre recombinase expression in in CD4 expressing T cells results in increased T-helper 17 cell differentiation.
|Allele Name||targeted mutation 2, Ronald M Evans|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||gf; Ppargflox; PPARgammaf; PPARgammafl|
|Gene Symbol and Name||Pparg, peroxisome proliferator activated receptor gamma|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A single loxP site was introduced upstream of exon 1 and a floxed neomycin cassette was inserted downstream of exon 2. The neomycin gene was excised from correctly targeted ES cells by transient transfection with a Cre expression plasmid.|
|Mutations Made By|| |
Yaacov Barak, University of Pittsburgh
When using the PPARγloxP mouse strain in a publication, please cite the originating article(s) and include JAX stock #004584 in your Materials and Methods section.
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