NOD.Cg-Tg(CD2-Ifngr2)2Pbro/DvsJ

Stock number: 004551
Product name: IFN-_γR2 TG
Strain synonyms: NOD.IFN-gRB Tg
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research

Description

These IFN-_γR2 mice express a transgene containing a sequence encoding mouse IFNGR2 and exhibit defects in Th1-dependent immunity in vivo as well as impaired Th1 polarization potential in vitro.

Detailed description

Expression of IFN-_γR2 in these TG mice is controlled by the CD2 promoter and enhancer. CD4⁺ T cells from IFN-_γR2 TG mice exhibit impaired Th1 polarization potential in vitro. TG mice also display several defects in Th1-dependent immunity in vivo, including attenuated delayed-type hypersensitivity responses and decreased antigen-specific IFN-_γ production. In addition, TG mice mount impaired Th1 responses against Leishmania major, as manifested by increased parasitemia and more severe lesions than their wild-type littermates. This strain is useful in studies related to the role of cytokine receptor expression in the development and function of T helper cells.

Development

The CD2-Ifngr2 construct was microinjected into (C57BL/6J x CBA/J)F1 oocytes and seven copies randomly inserted in this line. This transgene was then backcrossed onto C57BL/6J for at least 4 generations and imported into the laboratory of Dr. David Serreze at The Jackson Laboratory where it was subsequently backcrossed onto the NOD/ShiLtDvs background. The congenic strain was fixed homozygous for the transgene and embryos were later cyopreserved from homozygous males at generation N9F21 bred with NOD/ShiLtDvs females.

Allele

Symbol: Tg(CD2-Ifngr2)2Pbro
Name: transgene insertion 2, Paul B Rothman
MGI accession ID: MGI:3785869
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(CD2-Ifngr2)2Pbro
Marker name: transgene insertion 2, Paul B Rothman
Chromosome: UN
Strain of origin: (C57BL/6 x CBA/J)F1
Expressed genes: Ifngr2,interferon gamma receptor 2,mouse, laboratory
Molecular note: The sequence encoding mouse IFNGR2 was placed under the control of the human CD2 promoter and enhancer and this construct was microinjected into (C57BL/6 x CBA/J) oocytes. Seven copies integrated in this insertion.