NOD.Cg-B2m^tm1Unc Mcph1^{Tg(HLA-A2.1)1Enge} Tg(B2M)55Hpl/DvsJ

Stock number: 004548
Research areas: Hematological Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Metabolism Research, Research Tools

Development

NOD.B6-TgN(HLA-A2.1)1Enge/Dvs (Stock No. 003088 ) at N10 was bred to NOD.Cg-B2m^tm1Unc Tg(B2M)55Hpl/Dvs (Stock No. 003355 ) at N8, yielding NOD.Cg- TgN(HLA-A2.1)1Enge B2m^tm1Unc Tg(B2M)55Hpl/Dvs. Sibling mating was then used to fix the strain homozygous for the TgN(HLA-A2.1)1Enge and Tg(B2M)55Hpl transgenes and the B2m^tm1Unc gene.

Allele

Symbol: Tg(B2M)55Hpl
Name: transgene insertion 55, Hidde Ploegh
MGI accession ID: MGI:2159101
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(B2M)55Hpl
Marker name: transgene insertion 55, Hidde Ploegh
Chromosome: UN
Strain of origin: (CBA x C57BL/LiA)F2
Expressed genes: B2M,beta-2-microglobulin,human
Molecular note: A transgene containing the human B2M gene was shown to be expressed in spleen cells of mice carrying the transgene.

Allele

Symbol: B2m^tm1Unc
Name: targeted mutation 1, University of North Carolina
MGI accession ID: MGI:1857133
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: B2m
Marker name: beta-2 microglobulin
Chromosome: 2
Strain of origin: 129P2/OlaHsd
Molecular note: Insertion of a neomycin-resistance gene into the second exon.

Allele

Symbol: Mcph1^{Tg(HLA-A2.1)1Enge}
Name: transgene insertion 1, Victor H Engelhard
MGI accession ID: MGI:3056375
Generation method: Transgenic
Attributes: Null/Knockout; Inserted expressed sequence; Humanized sequence
Marker symbol: Mcph1
Marker name: microcephaly, primary autosomal recessive 1
Chromosome: 8
Strain of origin: C57BL/6
Expressed genes: HLA-A,major histocompatibility complex, class I, A,human
Molecular note: The transgene contains the full length HLA-A2.1 gene. Expression of human class I MHC Ag HLA-A2.1 was detected on cells from homozygous transgenic spleen, bone marrow, and thymus. Line 1 inserted into the gene at 18736683-18757058 (Build GRCm38/mm10) resulting in a duplication. The duplication results in a functional knock-out. Founder line 1 has a copy number of 3-8.
General note: Expression of this human class I molecule in transgenic mice does not result in expansion of the number of cytotoxic T lymphocyte (CTL) precursors specific for other human class I Ag, HLA-B27 or HLA-A2.2.