Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | B2m | beta-2 microglobulin |
Allele Type |
---|
Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Type |
---|
Transgenic (Null/Knockout, Inserted expressed sequence, Humanized sequence) |
NOD.B6-TgN(HLA-A2.1)1Enge/Dvs (Stock No. 003088
) at N10 was bred to NOD.Cg-B2mtm1Unc Tg(B2M)55Hpl/Dvs (Stock No. 003355
) at N8, yielding NOD.Cg- TgN(HLA-A2.1)1Enge B2mtm1Unc Tg(B2M)55Hpl/Dvs. Sibling mating was then used to fix the strain homozygous for the TgN(HLA-A2.1)1Enge and Tg(B2M)55Hpl transgenes and the B2mtm1Unc gene.
Expressed Gene | B2M, beta-2-microglobulin, human |
---|---|
Site of Expression | |
Expressed Gene | HLA-A, major histocompatibility complex, class I, A, human |
Site of Expression |
Allele Name | targeted mutation 1, University of North Carolina |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | b2mnull; beta2M-; beta2m0; beta2mnull; beta2mo; beta2mtm1Unc; beta2MKO; beta2-m-KO; I0; MHC-I- |
Gene Symbol and Name | B2m, beta-2 microglobulin |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 2 |
Molecular Note | Insertion of a neomycin-resistance gene into the second exon. |
Mutations Made By | Dr. Oliver Smithies, University of North Carolina at Chapel Hill |
Allele Name | transgene insertion 55, Hidde Ploegh |
---|---|
Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | (B2M)55HplTG |
Gene Symbol and Name | Tg(B2M)55Hpl, transgene insertion 55, Hidde Ploegh |
Gene Synonym(s) | |
Promoter | B2M, beta-2-microglobulin, human |
Expressed Gene | B2M, beta-2-microglobulin, human |
Strain of Origin | (CBA x C57BL/LiA)F2 |
Chromosome | UN |
Molecular Note | A transgene containing the human B2M gene was shown to be expressed in spleen cells of mice carrying the transgene. |
Allele Name | transgene insertion 1, Victor H Engelhard |
---|---|
Allele Type | Transgenic (Null/Knockout, Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Mcph1, microcephaly, primary autosomal recessive 1 |
Gene Synonym(s) | |
Promoter | HLA-A2.1, major histocompatibility complex, class I, subtype A2.1, human |
Expressed Gene | HLA-A, major histocompatibility complex, class I, A, human |
Strain of Origin | C57BL/6 |
Chromosome | 8 |
General Note | Expression of this human class I molecule in transgenic mice does not result in expansion of the number of cytotoxic T lymphocyte (CTL) precursors specific for other human class I Ag, HLA-B27 or HLA-A2.2. |
Molecular Note | The transgene contains the full length HLA-A2.1 gene. Expression of human class I MHC Ag HLA-A2.1 was detected on cells from homozygous transgenic spleen, bone marrow, and thymus. Line 1 inserted into the gene at 18736683-18757058 (Build GRCm38/mm10) resulting in a duplication. The duplication results in a functional knock-out. Founder line 1 has a copy number of 3-8. |
Mutations Made By | Dr. Ai-Xuan Le, Stanford University |
When using the NOD.Cg-B2mtm1Unc Mcph1Tg(HLA-A2.1)1Enge Tg(B2M)55Hpl/DvsJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #004548 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for B2m<tm1Unc>,Hemizygous or Non carrier for Tg(B2M)55Hpl, Hemizygous or Non carrier for Tg(HLA-A2.1)1Enge |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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