C57BL/6J-hlb156/J

Stock number: 004517
Strain synonyms: HLB156
Research areas: Hematological Research, Immunology, Inflammation and Autoimmunity Research

Description

These mice carry an ENU-induced mutation characterized by high neutrophil levels.

Detailed description

Mice that are homozygous for the mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Screening of third generation mice (G3) on standard chow identified a 6 week old male (born 5/27/2002) with a neutrophil level of 27.4%, which is approximately 130% higher than controls. This mutant mouse strain may be useful in studies of neutrophilia.

Development

Following multidose ethylnitrosourea (ENU) treatments to induce mutations in male founder C57BL/6J mice (Stock No. 000664), a forward genetic screen was utilized to identify phenotypic deviants in complex heart, lung, blood, and sleep disorders, at the Mouse Heart, Lung, Blood, and Sleep Disorders (HLBS) Center at The Jackson Laboratory. hlb156 was mapped by crossing to 129S1/SvImJ mice. Mapping identified the location of the mutation in an interval of 3.3cM on Chromosome 17, between D17Mit133 and D17Mit175.Heterozygotes were crossed to generate homozygotes. The mice have been maintained on a C57BL/6J background.

Allele

Symbol: hlb156
Name: heart, lung and blood, 156
MGI accession ID: MGI:2679518
Generation method: Chemically induced (ENU)
Marker symbol: hlb156
Marker name: heart, lung and blood, 156
Chromosome: 17
Strain of origin: C57BL/6J
Molecular note: This phenotypic mutant was identified in an ENU mutagenesis screen.