CD11c-DTR/GFP transgenic line 57 has the CD11c promoter (Itgax) directing expression of a diphtheria toxin receptor&nbsp;(designated&nbsp;heparin-binding EGF-like growth factor [HBEGF]) - enhanced green fluorescent protein (DTR/EGFP) fusion protein to dendritic cell populations. Line 57 has more than 20 copies of the CD11c-DTR/GFP transgene inserted into the 1700016L21Rik locus on chromosome 1 (80448681-80455738 bp [Build GRCm38/mm10]). Mice that are homozygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. Upon diphtheria toxin (DT) administration, mice harboring this transgene are transiently depleted of dendritic cell (DC) populations. All CD8+ and CD8- DC in the spleen express EGFP and are DT sensitive. Immunohistochemical and flow cytometric analysis reveals EGFP expression and DT-inducible depletion of CD11chigh DC in spleen, lymph node, lung, liver and lamina propria tissues, as well as the defined macrophage subpopulations of the alveolar, lamina propria, metallophillic and marginal zone. Rapid reduction of CD11c+ DC populations induced by intraperitoneal injection of DT persists for approximately 2 days, after which the cell population gradually recovers. While transient DC depletion was not associated with sign of illness or long-term defects, repeated DT induction is lethal to the mouse. Long term depletion of this cell population can be achieved by the generation of mixed irradiation chimeras. CD11c-DTR/GFP transgenic mice are useful in studies of mononuclear phagocyte origins and the specific role of dendritic cells in the immune response. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

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CD11c-DTR/GFP transgenic line 57 has the CD11c promoter (Itgax) directing expression of a diphtheria toxin receptor&nbsp;(designated&nbsp;heparin-binding EGF-like growth factor [HBEGF]) - enhanced green fluorescent protein (DTR/EGFP) fusion protein to dendritic cell populations. Administration of diphtheria toxin results in depletion of dendritic cell populations. CD11c-DTR/GFP transgenic mice are useful in studies of mononuclear phagocyte origins and the specific role of dendritic cells in the immune response. In <a href="https://www.ncbi.nlm.nih.gov/pubmed/30659012">Goodwin et al. 2019 Genome Res. 29:494</a>, it was discovered that line 57 has more than 20 copies of the CD11c-DTR/GFP transgene inserted into the 1700016L21Rik locus on chromosome 1 (80448681-80455738 bp [Build GRCm38/mm10]).

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