These Clock mice express a transgene containing a circular BAC 54 clone and hemizygotes exhibit a short circadian period.
Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr
Mice hemizygous for this transgene, a 140kb circular BAC 54 clone of the targeted gene, express 3-4 times more gene transcript than the wildtype. Hemizygous mice are viable, fertile, normal in size and do not display any gross physical abnormalities. Quantification of transgene copy number was estimated from Southern blot analysis. Hemizygotes exhibit a circadian period that is shorter than the wildtype. This transgene rescues the homozygous mutant Clock phenotype. This mutant mouse strain may be useful in studies of advanced sleep phase syndrome.
A transgenic construct consisting of a 140kb circular BAC 54 clone of the targeted gene was injected into fertilized oocytes that were a mix of CD1, C57BL/6J and BALB/cJ. Founder mice were intercrossed for four generations before transgenic females were backcrossed to C57BL/6 males to establish a congenic line.
|Expressed Gene||Clock, clock circadian regulator, rat|
|Site of Expression|
|Allele Name||transgene insertion 36, Joseph S Takahashi|
|Allele Type||Transgenic (Inserted expressed sequence)|
|Allele Synonym(s)||Clock gene BAC|
|Gene Symbol and Name||Tg(BAC54)36Jt, transgene insertion 36, Joseph S Takahashi|
|Gene Synonym(s)||Clock gene BAC|
|Promoter||Clock, clock circadian regulator, rat|
|Expressed Gene||Clock, clock circadian regulator, rat|
|Strain of Origin||CD-1 x (BALB/cJ x C57BL/6J)F2|
|Molecular Note||The transgene consists of a 140kb circular BAC 54 clone containing the Clock gene (circadian locomoter output cycles kaput), and includes the Clock promoter.|
|Mutations Made By|| |
Dr. Joseph Takahashi, Univ Texas Southwestern Medical Ctr
The resulting founder animals were crossed to C57BL/6 mice, and then backcrossed to the same for 8 generations.
When using the Clock mouse strain in a publication, please cite the originating article(s) and include JAX stock #004459 in your Materials and Methods section.
|Hemizygous or non carrier for Tg(BAC54)36Jt|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
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