B6.Cg-Tg(BAC54)36Jt/J

Stock number: 004459
Product name: Clock

Description

These Clock mice express a transgene containing a circular BAC 54 clone and hemizygotes exhibit a short circadian period.

Detailed description

Mice hemizygous for this transgene, a 140kb circular BAC 54 clone of the targeted gene, express 3-4 times more gene transcript than the wildtype. Hemizygous mice are viable, fertile, normal in size and do not display any gross physical abnormalities. Quantification of transgene copy number was estimated from Southern blot analysis. Hemizygotes exhibit a circadian period that is shorter than the wildtype. This transgene rescues the homozygous mutant Clock phenotype. This mutant mouse strain may be useful in studies of advanced sleep phase syndrome.

Development

A transgenic construct consisting of a 140kb circular BAC 54 clone of the targeted gene was injected into fertilized oocytes that were a mix of CD1, C57BL/6J and BALB/cJ. Founder mice were intercrossed for four generations before transgenic females were backcrossed to C57BL/6 males to establish a congenic line.

Allele

Symbol: Tg(BAC54)36Jt
Name: transgene insertion 36, Joseph S Takahashi
MGI accession ID: MGI:3057169
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(BAC54)36Jt
Marker name: transgene insertion 36, Joseph S Takahashi
Chromosome: UN
Strain of origin: CD-1 x (BALB/cJ x C57BL/6J)F2
Expressed genes: Clock,clock circadian regulator,rat
Molecular note: The transgene consists of a 140kb circular BAC 54 clone containing the Clock gene (circadian locomoter output cycles kaput), and includes the Clock promoter.