NOD.Cg-H2h4/DilTacUmm mice are completely protected from diabetes; however, a low incidence of perivascular and periductal insulitis is detected (20-30%). Protection from diabetes is complete even upon treatment with cyclophosphamide. These mice do, however, develop spontaneous thyroiditis and produce IgG autoantibodies when treated with 0.05% NaI in water (100% incidence in both sexes 6-8 week post treatment or by 4 months of age). Without treatment, thyroiditis is delayed and incomplete (60-70% incidence in 7-10 month old mice). This strain is an excellent model for autoimmune thyroiditis.
NOD/MrkTac were outbred to B10.A-H2h4/(4R)SgDvEg (Stock No. 001150), which carries the recombinant major histocompatibility H2h4 haplotype. The offspring were then backcrossed to NOD/MrkTac for five generations selecting for H2h4 at each generation. At N6, individuals heterozygous for the H2h4 MHC were intercrossed and resulting homozygous individuals were brother-sister mated.
When using the NOD.H-2h4 mouse strain in a publication, please cite the originating article(s) and include JAX stock #004447 in your Materials and Methods section.