B6.129S4-Ccl2^tm1Rol/J

Stock number: 004434
Product name: Ccl2 KO
Strain synonyms: MCP-1^-
Research areas: Cancer Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research

Description

Ccl2 KO mice, also known as MCP-1^- mice, have a neo cassette replacing exon 2 of the chemokine (C-C motif) ligand 2 (Ccl2) gene. This mutant mouse strain represents a model that may be useful in studies related to leukocyte trafficking.

Detailed description

Ccl2 KO mice, also known as MCP-1^- mice, have a neo cassette replacing exon 2 of the chemokine (C-C motif) ligand 2 (Ccl2) gene. No gene product is detected in lipopolysaccharide (LPS)-stimulated peritoneal macrophages isolated from homozygous mice. The numbers of peritoneal macrophages, Kupffer cell and alveolar macrophages were similar to levels found in wildtype mice. Thioglycollate induced peritonitis results in impaired recruitment of monocytes and macrophages to peritoneal cavity. Cellular recruitment to delayed-type hypersensitivity challenges and secondary granulomata is reduced. Mice that are homozygous for the targeted mutation are viable and fertile.

Development

A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 2 and insert an in frame stop codon into exon 1 of the chemokine (C-C motif) ligand 2 (Ccl2) gene. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6 for ten generations by the donating lab. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes. (Stock No. 000664).

Allele

Symbol: Ccl2^tm1Rol
Name: targeted mutation 1, Barrett J Rollins
MGI accession ID: MGI:2175911
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Ccl2
Marker name: chemokine (C-C motif) ligand 2
Chromosome: 11
Strain of origin: 129S4/SvJae
Molecular note: A small deletion and an in frame stop codon were created in exon 1. A PGK-neomycin resistance cassette was also inserted into exon 2. Secreted protein was not detected in stimulated peritoneal macrophages from homozygous mutant mice.