C.Cg-Tcra^tm1Mom Tcrb^tm1Mom/J

Stock number: 004364
Product name: TCRaKO bKO
Research areas: Hematological Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These Tcra and Tcrb double knock-out mice may be useful in studies related to production of the immunoglobulin (Ig)E class of antibodies in the pathogenesis of allergies.

Detailed description

Mice that are homozygous for the targeted mutation are viable and fertile. Mutant mice display early stage arrest of alpha beta thymocyte differentiation. Mice may develop inflammatory bowel disease.

Development

For details related to the construction of each allele, please refer to the entries for strains 002116 and 002118.

Allele

Symbol: Tcrb^tm1Mom
Name: targeted mutation 1, Peter Mombaerts
MGI accession ID: MGI:1857256
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Tcrb
Marker name: T cell receptor beta chain
Chromosome: 6
Strain of origin: 129P2/OlaHsd
Molecular note: 15kb of sequence was replaced with a PGK-neo cassette. The deletion included one of the two D gene segments, ten of the twelve J gene segments, and both constant gene segments.

Allele

Symbol: Tcra^tm1Mom
Name: targeted mutation 1, Peter Mombaerts
MGI accession ID: MGI:1857255
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Tcra
Marker name: T cell receptor alpha chain
Chromosome: 14
Strain of origin: 129S2/SvPas
Molecular note: A PGK-neo cassette was used to disrupt exon 1, which encodes the majority of the extracellular domain. The targeting event was confirmed by Southern analysis.