This strain is one of a set of 9 NOD.B10 congenic Idd5 strains (Stock No. 008893, 008894, 012391, 012392, 012394, 012393, 012395, and 012396 that resulted by crossing segments of C57BL/10SnJ derived chromosome 1 into the NOD/MrkTac background. These strains may be useful to better understand the variation of diabetic resistance conferred by Idd loci; for studying long-range chromosome remodeling and may be useful for identifying diabetes susceptible candidate genes within the Idd5 subloci.
Dr. David Serreze, The Jackson Laboratory
This NOD/MrkTac congenic strain, commonly referred to as NOD.B10-Idd5R444, line 1094 contains a 19.4Mb genomic segment from the diabetes resistant strain C57BL/10SnJ (Stock No. 000666) introgressed into chromosome 1 and is defined within markers D1Mit124 (57.7Mb) and D1Mit124 (77.1Mb). The C57BL/10SnJ genomic segment includes Idd5.1, Idd5.3 and Idd5.2. The major candidate genes for Idd5.1 and Idd5.2 are Ctla4 and Nramp1, respectively. The Idd5.3 is defined to a 3.55Mb region. Within this region there are 11 annotated genes; including possible candidate genes IKAROS family zinc finger 2 (Ikzf2) and acyl-Coenzyme A dehydrogenase, long-chain (Acadl).
Diabetes incidence among 7-month old, significantly protected, females is reported to be 13%, compared with more than 80% in NOD controls or 62% in NOD.B10-Idd5.1 (Stock No. 012391) and NOD.B10-Idd5.1, Idd5.3 (Stock No. 012392) (Hunter, et al. 2007.
This strain may be useful for positional mapping of the Idd5.3 loci; to identify candidate genes within the Idd5.3 region and to understand the variation in diabetic resistance conferred by Idd loci, in particular the epistatic interactions of Idd5.1 and Idd5.2 alleles with alleles at Idd5.3
This strain was produced by mating mice from NOD/MrkTac (NOD) to C57BL/10SnJ (Stock No. 000666) followed by 12 additional crosses to NOD (N13) with selection for chromosome 1, Idd5 markers. Siblings carrying the C57BL/10SnJ derived segments denoted by markers D1Mit124 (57.7Mb) through D1Mit132 (77.1Mb) were intercrossed to make homozygous. NCBI based 5kSNP Chip Data identified no C57BL/10SnJ genetic contamination of other chromosomes. The Type 1 Diabetes Resource received this strain in 2010.
|Gene Symbol and Name||Idd5, insulin dependent diabetes susceptibility 5|
|Strain of Origin||C57BL/10SnJ|
|General Note||Idd3 and Idd5 appear to interact additively. C57BL/6-derived alleles at Idd3 in combination with C57BL/10SnJ-derived alleles at Idd5 partially reverses autoimmune exocrinopathy markers on an NOD genetic background.|
|Molecular Note||This allele confers resistance to insulin dependent diabetes compared to NOD/MrkTac.|