When bred to a strains expressing Cre recombinase under the control of a tetracycline-responsive promoter element and expressing a tetracycline-controlled activator protein in brain tissues or neuronal cells, for example, this mutant mouse strain may be useful in studies of hippocampal-dependent learning and long-term potentiation, and epilepsy respectively.
Rudolf Jaenisch, Whitehead Institute, Massachusetts Institute of Technology
These mice possess loxP sites on either side of the brain derived neurotrophic factor (Bdnf) coding region. Mice that are homozygous for this allele are viable and fertile.
When bred to a strain expressing Cre recombinase under the control of a tetracycline-responsive promoter element (see Stock No. 006224, 006234, 006244) and a strain expressing a tetracycline-controlled activator protein in brain tissues (see Stock No. 003763), this mutant mouse strain may be useful in studies of hippocampal-dependent learning and long-term potentiation.
When bred to a strain expressing Cre recombinase in neuronal cells (see Stock No. 003966 for example), this mutant mouse strain may be useful in studies of epilepsy.
A targeting vector was designed to insert a single loxP site upstream of the coding region, and a loxP flanked hygromycin resistance (hygro) cassette downstream of the coding region of the brain derived neurotrophic factor (Bdnf) gene. This construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells which were transiently transfected with a Cre-recombinase vector to remove the selection cassette. Correctly targeted ES cells were injected into BALB/c blastocysts.
|Allele Name||targeted mutation 3, Rudolf Jaenisch|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Bdnflox; Bdnf2; Bdnf2L; Bdnf2lox; Bdnftm2Jae|
|Gene Symbol and Name||Bdnf, brain derived neurotrophic factor|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A single loxP site was inserted 5' to the only coding exon, and a loxP-flanked hygromycin resistance cassette was inserted 3' to the exon. The hygromycin selection cassette was removed in ES cells by transient Cre expression leaving a single loxP site prior to the production of chimeric mice.|
This strain originated on a mixed C;129S4 background. The Donating Investigator maintained it as as a homozygote on a mixed B6, 129S4, BALB/c background. Coat color expected from breeding:Albino
When using the Bdnf2lox mouse strain in a publication, please cite the originating article(s) and include JAX stock #004339 in your Materials and Methods section.