These congenic mice carry the Tyr+ allele from CBA/JLsLt and are agouti in color making them suitable blastocyst donors for microinjection of NOD ES cells.
Dr. Edward Leiter, The Jackson Laboratory
This Chr 7 congenic strain in which the tyrosinase allele Tyr+ of the CBA/JLsLt strain is fixed on the NOD/Lt background, therefore making the strain agouti in color. This strain should be a good donor for blastocysts in which to microinject targeted NOD ES cells, and may alleviate unwanted genome integration experienced with use of the C57BL/6 blastocysts. The congenic segment on Chromosome 7 slightly supresses spontaneous T1D development which is an asset when these females are used as blastocyst recipients.
A CBcNO6/Lt (Stock No. 002349) derived, Chr 7 genomic segment containing Tyr+ and extending from D7Mit250 to D7Mit66 was transferred to NOD/Lt for 9 generations. Marker assisted analysis indicates all known Idd are of NOD/Lt origin. In 2005, NOD.CBALs-Tyr+/Lt was transferred to the Type 1 Diabetes Resource at The Jackson Laboratory.
When using the NOD.Agouti mouse strain in a publication, please cite the originating article(s) and include JAX stock #004304 in your Materials and Methods section.