NOD.129P2(Cg)-Il10^tm1Cgn Il4^tm1Cgn/DvsJ

Stock number: 004291
Product name: NOD.IL4/10^null
Research areas: Cancer Research, Diabetes and Obesity Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research

Description

These double knock-out mice develop type 1 diabetes at a rate equivalent to the parental NOD strain.

Detailed description

Mice homozygous for the Il10 and Il4 targeted mutations are viable and fertile when housed under SPF conditions. NOD/Lt mice deficient for both of these genes develop type 1 diabetes at a rate equivalent to the parental strain. The Il10 ^tm1Cgn mutation also renders this NOD/Lt stock susceptible to colitis (although not as severe as other strains of Il10 deficient mice) when maintained under standard housing conditions.

Development

For details related to the construction of each allele, please refer to the entries for strains 004266 and 004222.

Allele

Symbol: Il4^tm1Cgn
Name: targeted mutation 1, University of Cologne
MGI accession ID: MGI:1857194
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Il4
Marker name: interleukin 4
Chromosome: 11
Strain of origin: 129P2/OlaHsd
Molecular note: A translational stop codon and a neomycin resistance gene were inserted into the first exon of the gene.

Allele

Symbol: Il10^tm1Cgn
Name: targeted mutation 1, University of Cologne
MGI accession ID: MGI:1857199
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Il10
Marker name: interleukin 10
Chromosome: 1
Strain of origin: 129P2/OlaHsd
Molecular note: A 500 bp genomic fragment containing codons 5-55 was replaced with a linker containing a termination codon followed by a neomycin cassette. A termination codon was also introduced into exon 3. No IL10 activity was detectable by ELISA assays in supernatants of in vitro cultures of Con A-stimulated splenic T cells derived from homozygous mice following infection with the nematode N. brasiliensis.