This strain was developed to provide humanized islet allograft donors. NOD-scid-HLA-A2 positive islet grafts in diabetic NOD-RagInullPrf1null mice are rejected by HLA-A2-negative, but not by HLA-A2-positive, human peripheral blood mononuclear cells. Graft rejection occurred in the absence of detectable graft-vs-host disease (GVHD). This strain is useful as a model for the in vivo study of human islet allograft rejection.
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Spontaneous | Prkdc | protein kinase, DNA activated, catalytic polypeptide |
Allele Type |
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Transgenic (Null/Knockout, Inserted expressed sequence, Humanized sequence) |
This strain was developed to provide humanized islet allograft donors. NOD-scid-HLA-A2 positive islet grafts in diabetic NOD-RagInullPrf1null mice are rejected by HLA-A2-negative, but not by HLA-A2-positive, human peripheral blood mononuclear cells. Graft rejection occurred in the absence of detectable graft-vs-host disease (GVHD). This strain is useful as a model for the in vivo study of human islet allograft rejection.
NOD.CB17-Prkdcscid was crossed once with NOD.B6-TgN(HLA-A2.1)1Enge/Dvs (Stock #003088) and the doubly heterozygous offspring were backcrossed once to NOD.CB17-Prkdcscid to make the strain homozygous for Prkdcscid then sibling mated to fix the strain homozygous for TgN(HLA-A2.1)1Enge as well.
Expressed Gene | HLA-A, major histocompatibility complex, class I, A, human |
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Site of Expression |
Allele Name | severe combined immunodeficiency |
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Allele Type | Spontaneous |
Allele Synonym(s) | SCID |
Gene Symbol and Name | Prkdc, protein kinase, DNA activated, catalytic polypeptide |
Gene Synonym(s) | |
Site of Expression | T and B lymphocytes. |
Strain of Origin | C.BKa-Ighb/Icr |
Chromosome | 16 |
Molecular Note | A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*). |
Allele Name | transgene insertion 1, Victor H Engelhard |
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Allele Type | Transgenic (Null/Knockout, Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Mcph1, microcephaly, primary autosomal recessive 1 |
Gene Synonym(s) | |
Promoter | HLA-A2.1, major histocompatibility complex, class I, subtype A2.1, human |
Expressed Gene | HLA-A, major histocompatibility complex, class I, A, human |
Strain of Origin | C57BL/6 |
Chromosome | 8 |
General Note | Expression of this human class I molecule in transgenic mice does not result in expansion of the number of cytotoxic T lymphocyte (CTL) precursors specific for other human class I Ag, HLA-B27 or HLA-A2.2. |
Molecular Note | The transgene contains the full length HLA-A2.1 gene. Expression of human class I MHC Ag HLA-A2.1 was detected on cells from homozygous transgenic spleen, bone marrow, and thymus. Line 1 inserted into the gene at 18736683-18757058 (Build GRCm38/mm10) resulting in a duplication. The duplication results in a functional knock-out. Founder line 1 has a copy number of 3-8. |
Mutations Made By | Dr. Ai-Xuan Le, Stanford University |
When using the NOD-scid-HLA-A2 mouse strain in a publication, please cite the originating article(s) and include JAX stock #004262 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous for Tg(HLA-A2.1)1Enge |
Frozen Mouse Embryo | NOD.Cg-Mcph1<Tg(HLA-A2.1)1Enge> Prkdc<scid>/Dvs Frozen | $2595.00 |
Frozen Mouse Embryo | NOD.Cg-Mcph1<Tg(HLA-A2.1)1Enge> Prkdc<scid>/Dvs Frozen | $2595.00 |
Frozen Mouse Embryo | NOD.Cg-Mcph1<Tg(HLA-A2.1)1Enge> Prkdc<scid>/Dvs Frozen | $3373.50 |
Frozen Mouse Embryo | NOD.Cg-Mcph1<Tg(HLA-A2.1)1Enge> Prkdc<scid>/Dvs Frozen | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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