NOD.B6-Tg(KLK4mHEL)6Ccg/Dvs

Stock number: 004258
Product name: KLK3

Detailed description

These KLK3 (KLK3 and KLK4 are synonymous) mice express a transgene containing the chicken lysozyme gene (HEL). Cells from spleen, lymph node, bone marrow, and thymus display membrane fluorescence in fluorescence activated cell sorting (FACS).

Development

Tg(KLK4mHEL) was backcrossed from C57BL/6-Tg(KLK4mHEL)6Ccg (stock#002598) onto NOD/Lt in the laboratory of Dr. David Serreze. In 2004 this strain reached N13.

Allele

Symbol: Tg(KLK4mHEL)6Ccg
Name: transgene insertion 6, Christopher C Goodnow
MGI accession ID: MGI:2384632
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(KLK4mHEL)6Ccg
Marker name: transgene insertion 6, Christopher C Goodnow
Chromosome: UN
Strain of origin: C57BL/6JSfd
Expressed genes: HEL,HEL,chicken
Molecular note: A genomic fragment containing the coding exons of the chicken lysozyme gene (also known as hen egg lysozyme, HEL) was linked to the ubiquitously-active mouse H2-K^b promoter and transmembrane sequence (which included the extracellular spacer sequence, transmembrane segment, and cytoplasmic tail). Fluorescence activated cell sorting (FACS) analysis of cells from spleen, lymph node, bone marrow, and thymus from mice carrying the transgene showed membrane expression of chicken lysozyme on these cells.