NOD.Cg-Prkdc^scid Tg(Ins2-E3)1Dvs/DvsJ

Stock number: 004230
Product name: RIP-E3/NOD-scid
Research areas: Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools, Virology Research

Description

These RIP-E3/NOD-scid mice express a transgene containing adenovirus early region 3 genes under control of the rat insulin II promoter (RIP).

Detailed description

Transgenic expression of adenovirus early region 3 genes under the rat insulin II promoter (RIP) in beta-cells in nonobese diabetic (NOD) mice (Stock No. 003869) decreases the incidence and delays the onset of autoimmune diabetes, but whether also true for these RIP-E3/NOD-scid mice has not been reported.

Development

The Tg(Ins2-E3)1Dvs transgene was introduced into B6D2F2 Oocytes and backcrossed onto C57BL/6J before then being backcrossed onto NOD.CB17-Prkdc^scid until N11 and then bred to homozygosity for Prkdc^scid and the transgene. This strain is maintained by sibling mating double homozygotes.

Allele

Symbol: Tg(Ins2-E3)1Dvs
Name: transgene insertion 1, David V Serreze
MGI accession ID: MGI:3785598
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Ins2-E3)1Dvs
Marker name: transgene insertion 1, David V Serreze
Chromosome: UN
Strain of origin: (C57BL/6J x DBA/2J)F2
Expressed genes: E3,adenovirus early region 3,adenovirus
Molecular note: The adenovirus early region 3 genes, from nucleotide 27752 to 31076, were put under the control of the rat insulin promoter.

Allele

Symbol: Prkdc^scid
Name: severe combined immunodeficiency
MGI accession ID: MGI:1857113
Generation method: Spontaneous
Marker symbol: Prkdc
Marker name: protein kinase, DNA activated, catalytic polypeptide
Chromosome: 16
Strain of origin: C.BKa-Igh^b/Icr
Site of expression: T and B lymphocytes.
Molecular note: A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).