NOD.Cg-Il10^tm1Cgn Il4^tm1Cgn/Dvs

Stock number: 004228
Research areas: Cancer Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research

Description

A subline of this strain has been cryopreserved. Please see Stock No. 004291

Development

The homozygous strains NOD.Cg-Il10^tm1Cgn/Dvs at N8 and NOD.129P2(B6)-Il4^tm1Cgn/Dvs at N9 were crossed together then sibling mated to make a strain homozygous for Il10^tm1Cgn and Il4^tm1Cgn considered to be the equivalent of N8 on the NOD background.

Allele

Symbol: Il4^tm1Cgn
Name: targeted mutation 1, University of Cologne
MGI accession ID: MGI:1857194
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Il4
Marker name: interleukin 4
Chromosome: 11
Strain of origin: 129P2/OlaHsd
Molecular note: A translational stop codon and a neomycin resistance gene were inserted into the first exon of the gene.

Allele

Symbol: Il10^tm1Cgn
Name: targeted mutation 1, University of Cologne
MGI accession ID: MGI:1857199
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Il10
Marker name: interleukin 10
Chromosome: 1
Strain of origin: 129P2/OlaHsd
Molecular note: A 500 bp genomic fragment containing codons 5-55 was replaced with a linker containing a termination codon followed by a neomycin cassette. A termination codon was also introduced into exon 3. No IL10 activity was detectable by ELISA assays in supernatants of in vitro cultures of Con A-stimulated splenic T cells derived from homozygous mice following infection with the nematode N. brasiliensis.