NOD/ShiLtDvs-Tg(Ins2-E3*309)5Dvs/DvsJ

Stock number: 004226
Product name: DL309/NOD
Research areas: Diabetes and Obesity Research

Description

These DL309/NOD mice express a transgene containing only the E3 gp19K gene of the adenovirus early region 3 genes under control of the rat insulin II promoter (RIP). Autoimmune type 1 diabetes (T1D) is weakly inhibited in these mice.

Detailed description

Autoimmune type 1 diabetes (T1D) is inhibited in NOD mice transgenically expressing all E3 genes under control of a rat insulin promoter (RIPE3/NOD, Stock No. 004320). For dissecting the protective mechanisms afforded by various E3 genes, they were subdivided into RIP-driven transgene constructs. Strong T1D protection mediated at the beta-cell level characterized DL704/NOD mice (Stock No. 004227) lacking the E3 gp19K gene suppressing MHC class I expression but retaining the 10.4K, 14.5K, and 14.7K genes inhibiting Fas- or TNF-alpha-induced apoptosis and TNF-alpha-induced NF-kB activation. Much weaker protection characterized DL309/NOD mice expressing the gp19K but not the 10.4K, 14.5K, and 14.7K genes. While RIPE3/NOD splenocytes had an unexpected decrease in ability to adoptively transfer T1D, splenocytes from both the DL704 and DL309 stocks efficiently did so.

Allele

Symbol: Tg(Ins2-E3*309)5Dvs
Name: transgene insertion 5, David V Serreze
MGI accession ID: MGI:3785516
Generation method: Transgenic
Attributes: Inserted expressed sequence
Synonyms: DL309; RIPE3DL309
Marker symbol: Tg(Ins2-E3*309)5Dvs
Marker name: transgene insertion 5, David V Serreze
Marker synonyms: DL309; RIPE3DL309
Chromosome: UN
Strain of origin: NOD/ShiLtDvs
Expressed genes: E3,adenovirus early region 3,adenovirus
Molecular note: The rat insulin promoter was placed upstream of a mutant adenovirus early region (E3) sequences with a 750bp deletion that disrupts the 10.4K, 14.5K, and 14.7K genes, and a 6bp deletion in the 6.7K gene, leaving the gp19K and 11.6K genes expressed.