These DL309/NOD mice express a transgene containing only the E3 gp19K gene of the adenovirus early region 3 genes under control of the rat insulin II promoter (RIP). Autoimmune type 1 diabetes (T1D) is weakly inhibited in these mice.
Read More +Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Autoimmune type 1 diabetes (T1D) is inhibited in NOD mice transgenically expressing all E3 genes under control of a rat insulin promoter (RIPE3/NOD, Stock No. 004320). For dissecting the protective mechanisms afforded by various E3 genes, they were subdivided into RIP-driven transgene constructs. Strong T1D protection mediated at the beta-cell level characterized DL704/NOD mice (Stock No. 004227) lacking the E3 gp19K gene suppressing MHC class I expression but retaining the 10.4K, 14.5K, and 14.7K genes inhibiting Fas- or TNF-alpha-induced apoptosis and TNF-alpha-induced NF-kB activation. Much weaker protection characterized DL309/NOD mice expressing the gp19K but not the 10.4K, 14.5K, and 14.7K genes. While RIPE3/NOD splenocytes had an unexpected decrease in ability to adoptively transfer T1D, splenocytes from both the DL704 and DL309 stocks efficiently did so.
Expressed Gene | E3, adenovirus early region 3, adenovirus |
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Site of Expression |
Allele Name | transgene insertion 5, David V Serreze |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | DL309; RIPE3DL309 |
Gene Symbol and Name | Tg(Ins2-E3*309)5Dvs, transgene insertion 5, David V Serreze |
Gene Synonym(s) | |
Promoter | Ins2, insulin 2, rat |
Expressed Gene | E3, adenovirus early region 3, adenovirus |
Strain of Origin | NOD/ShiLtDvs |
Chromosome | UN |
Molecular Note | The rat insulin promoter was placed upstream of a mutant adenovirus early region (E3) sequences with a 750bp deletion that disrupts the 10.4K, 14.5K, and 14.7K genes, and a 6bp deletion in the 6.7K gene, leaving the gp19K and 11.6K genes expressed. |
When using the DL309/NOD mouse strain in a publication, please cite the originating article(s) and include JAX stock #004226 in your Materials and Methods section.
Facility Barrier Level Descriptions
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Hemizygous or Non carrierfor Tg(Ins2-E3*309)5Dvs |
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