B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J

Stock number: 004218
Product name: CK6/ECFP
Research areas: Research Tools

Description

All tissues from hemizygous animals, with the exception of erythrocytes and adipocytes, display fluorescence in all cell types under appropriate lighting conditions.

Detailed description

Mice that are hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. All tissues from hemizygous animals display fluorescence in all cell types under appropriate lighting conditions. Notable exceptions to this phenotype are erythrocytes and adipocytes in which fluorescence is negligible or absent.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A transgenic construct containing an Enhanced Cyan Fluorescent Protein gene (Clontech) under the control of the a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer, was introduced into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. ES cells containing the construct were aggregated with ICR morulae and the resulting chimeric males were bred with ICR females for germline transmission. The transgenic offspring were intercrossed to generate homozygotes. Mice derived from founder line CK6/ECFP were sent to The Jackson Laboratory in 2000 and used to establish the colony on a mixed genetic background (see Stock No. 003773). From there, some mice were then backcrossed for more than 5 generations to C57BL/6J mice to establish this congenic strain.

Allele

Symbol: Tg(CAG-ECFP)CK6Nagy
Name: transgene insertion CK6, Andras Nagy
MGI accession ID: MGI:3497912
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(CAG-ECFP)CK6Nagy
Marker name: transgene insertion CK6, Andras Nagy
Chromosome: UN
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Expressed genes: CFP,Cyan Fluorescent Protein,jellyfish
Site of expression: All tissues from hemizygous animals display fluorescence in all cell types under appropriate lighting conditions. Notable exceptions to this phenotype are erythrocytes and adipocytes, in which fluorescence is negligible or absent.
Molecular note: The transgene construct contains an enhanced cyan fluorescent protein gene driven by the cytomegalovirus (CMV) immediate early enhancer coupled to the chicken beta actin promoter.
General note: Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. All tissues from these animals display fluorescence in all cell types under appropriate lighting conditions, except in erythrocytes and adipocytes where fluorescence is negligible or absent.