These Itgb5 knock-out mice exhibit defects in vascular endothelial growth factor (VEGF)-mediated vascular permeability.
Dean Sheppard, Univ of California Medical Center (UCSF)
Mice that are homozygous for the Itgb5tm1Des targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. No Itgb5 gene product (mRNA or protein) is detected. Homozygotes display defects in VEGF-mediated vascular permeability. Cultured keratinocytes derived from homozygous mutant animals display impaired adhesion and migration on vitronectin-coated surfaces.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt a region of the Itgb5 gene corresponding to human amino acids 114-230. The construct was electroporated into 129S4/SvJae-derived RF8 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were mated to an unspecified strain of 129 mice.
|Allele Name||targeted mutation 1, Dean Sheppard|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Itgb5, integrin beta 5|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A neomycin resistance cassette replaced part of an exon of the gene that corresponds to amino acids 114 to 230 in the reported human sequence, and an adjacent intron. Northern analysis using a murine cDNA probe did not detect transcript in cultured keratinocytes from homozygous mutant mice. Immunoprecipitation studies using antiserum raised against the cytoplasmic domain of human did not detect protein in lysates of cultured keratinocytes from homozygous mutant mice.|
|Mutations Made By|| |
Xiaozhu Huang, Univ of California Medical Center (UCSF)
This strain originated from a chimeric mouse on a B6;129S4 background which was mated to an unspecified 129 strain once before being made homozygous.
When using the Β5- mouse strain in a publication, please cite the originating article(s) and include JAX stock #004166 in your Materials and Methods section.