These UAS-lacZ mice express a transgene containing five upstream activating sequence (UAS) elements, a Wnt1 minimal promoter, lacZ, and an SV40 poly A sequence.
David H. Rowitch, University of California, San Francisco
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Expression of the transgenic lacZ gene is directed by upstream activating sequence (UAS) elements. This strain serves as a reporter for mice employing GAL4/UAS bigenic system for controlled gene expression in the developing CNS. In this system, a transgenic strain expressing the yeast transcriptional activator GAL4 (see Stock No. 003829) is bred to a second transgenic, target strain bearing an UAS-regulated gene. In the double transgenic offspring, an UAS-regulated gene would be selectively expressed in tissues expressing GAL4.
A transgenic construct containing five UAS elements, a Wnt1 minimal promoter, lacZ and an SV40 poly A sequence was injected into fertilized B6CBAF1/J mouse eggs. Founder animals were obtained and made homozygous.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | This strain serves as a reporter for mice employing the GAL4/UAS bigenic system for controlled gene expression. |
Allele Name | transgene insertion 65, David H Rowitch |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(UAS-lacZ)65Rth, transgene insertion 65, David H Rowitch |
Gene Synonym(s) | |
Promoter | UAS, upstream activating sequence, yeast |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | This strain serves as a reporter for mice employing the GAL4/UAS bigenic system for controlled gene expression. |
Strain of Origin | (C57BL/6J x CBA/J)F1 |
Chromosome | UN |
General Note | Homozygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. |
Molecular Note | The transgene contains five upstream activating sequence (UAS) elements, a Wnt1 minimal promoter, lacZ, and an SV40 poly A sequence. UAS elements drive expression of the transgenic lacZ gene in transgenic mice. |
Mutations Made By | David Rowitch, University of California, San Francisco |
This strain originated on a B6CBAF1/J background. Hemizygous mice were mated to generate homozygotes. Expected coat color is: Black, and Agouti.
When using the UAS-lacZ mouse strain in a publication, please cite the originating article(s) and include JAX stock #004141 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(UAS-lacZ)65Rth |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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