B6.SOD1-Sod1^c/CjeDnJ

Stock number: 004140
Research areas: Research Tools

Description

The Sod1^c polymorphism is useful as a genetic marker.

Detailed description

The Sod1^c polymorphism has a G to A transition at position 231 changing codon 77, within a positively charged amino acid domain of the Zinc subloop, from glutamate to lysine. The isoelectric focusing point is calculated to be 6.85 instead of 6.50. CuZn SOD activity in whole brain homogenates is 11% less in Sod1^c homozygotes than in Sod1^a homozygotes, and heterozygotes have in intermediate level of activity. No abnormal phenotype has been detected. This polymorphism is useful as a genetic marker.

Development

The Sod1^c allele was backcrossed from the strain SOD1/EiJ onto the C57BL/6J background by Charles Epstein at UCSF. In 2000 homozygous females at backcross generation N10 and homozygous males at either backcross generation N10 or N11 were shipped to Muriel Davisson at The Jackson Laboratory and this strain was sibling inbred from that point on until 2005 when embryos were generated for cryopreservation by in vitro fertilization using C57BL/6J females and homozygous males at generation N10+F11.

Allele

MGI accession ID: MGI:98351
Marker symbol: Sod1
Marker name: superoxide dismutase 1, soluble
Chromosome: 16