These Il18r1 knock-out mice have reduced binding of IL-18 on the surface of T helper cells (Th1). They have decreased levels of interferon gamma production in response to IL-18, diminished natural killer cell activity, and impaired T helper lymphocyte response.
Shizuo Akira, Osaka University
Genetic Background | Generation |
---|---|
N13F3
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Il18r1 | interleukin 18 receptor 1 |
Mice that are homozygous null for the Il18r1 gene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No Il18r1 mRNA is detected. Homozygous Il18r1 null mice are similar in phenotype to IL-18-deficient mice. They show reduced binding of IL-18 on the surface of Th1 cells, and exhibit decreased levels of interferon gamma production in response to IL-18. Natural killer cell activity is also diminished and the T helper lymphocyte response in impaired.
A targeting vector containing neomycin resistance and Herpes simplex virus thymidine kinase genes was used to disrupt an exon encoding the transmembrane domain (amino acids 296-350). The construct was transfected into 129P2/OlaHsd-derived E14-1 embryonic stem cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were backcrossed to C57BL/6 mice.
Allele Name | targeted mutation 1, Shizuo Akira |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Il18r1-; Il18ralpha-; Il1rrptm1Aki |
Gene Symbol and Name | Il18r1, interleukin 18 receptor 1 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 1 |
Molecular Note | Replacement of the exon encoding amino acids 296-350, encoding the transmembrane region, with a neomycin cassette. No mRNA was detected in liver from homozygous mutant animals, as assayed by RT-PCR. |
Mutations Made By | Shizuo Akira, Osaka University |
This strain originated on a B6;129P2 background and has been backcrossed to C57BL/6 for at least 6 generations (7/01). Coat color expected from breeding:Black
When using the IL-1Rrp KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #004131 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Il18r1<tm1Aki> |
Frozen Mouse Embryo | B6.129P2-Il18r1<tm1Aki>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Il18r1<tm1Aki>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Il18r1<tm1Aki>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129P2-Il18r1<tm1Aki>/J Frozen Embryos | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.