B6.129P2-Il18r1^tm1Aki/J

Stock number: 004131
Product name: IL-1Rrp KO
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These Il18r1 knock-out mice have reduced binding of IL-18 on the surface of T helper cells (Th1). They have decreased levels of interferon gamma production in response to IL-18, diminished natural killer cell activity, and impaired T helper lymphocyte response.

Detailed description

Mice that are homozygous null for the Il18r1 gene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No Il18r1 mRNA is detected. Homozygous Il18r1 null mice are similar in phenotype to IL-18-deficient mice. They show reduced binding of IL-18 on the surface of Th1 cells, and exhibit decreased levels of interferon gamma production in response to IL-18. Natural killer cell activity is also diminished and the T helper lymphocyte response in impaired.

Development

A targeting vector containing neomycin resistance and Herpes simplex virus thymidine kinase genes was used to disrupt an exon encoding the transmembrane domain (amino acids 296-350). The construct was transfected into 129P2/OlaHsd-derived E14-1 embryonic stem cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were backcrossed to C57BL/6 mice.

Allele

Symbol: Il18r1^tm1Aki
Name: targeted mutation 1, Shizuo Akira
MGI accession ID: MGI:2136765
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Il18r1
Marker name: interleukin 18 receptor 1
Chromosome: 1
Strain of origin: 129P2/OlaHsd
Molecular note: Replacement of the exon encoding amino acids 296-350, encoding the transmembrane region, with a neomycin cassette. No mRNA was detected in liver from homozygous mutant animals, as assayed by RT-PCR.