The mutants develop detectable head-tilt and slight tremor by weaning age (average onset 16 +/- 3 days). Mutants of either gender have been produced and homozygotes are viable. Detailed whole ear histology on 1 mutant (age 48 days) showed saccular and utricular otoconia; no labyrinth malformation was observed. Standard pathology work-up on 3 other mutants (age 20 days) showed the thymus had a thin, atrophic cortex with few lymphocytes and some apoptosis; the spleen was very small. Standard eye histology on one mutant and one control littermate (age 29 days) showed no abnormalities.
This phenotypic deviant was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males (Stock No. 000664), in the Neuroscience Mutagenesis facility at The Jackson Laboratory. Mutagenized males were crossed to C57BL/6J females; G3 descendants of the mutagenized males were selected for neurological impairment.
|Allele Name||neuroscience mutagenesis facility, 4|
|Allele Type||Chemically induced (ENU)|
|Gene Symbol and Name||nmf4, neuroscience mutagenesis facility, 4|
|Strain of Origin||C57BL/6J|
|Molecular Note||This phenotypic mutant was identified in an ENU mutagenesis screen.|