C3.129P2(B6)-B2m^tm1Unc/Dcr

Stock number: 004040
Product name: Β₂M-
Research areas: Hematological Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Metabolism Research, Research Tools

Description

These B2m knock-out mice exhibit a severely deficient immune response involving CD8⁺ T-cells.

Detailed description

Mice homozygous for the B2m^tm1Unc targeted mutation have little if any MHC class I protein expression on the cell surface. There are few CD4- CD8+ cytotoxic T-cells and under some circumstances there is a compensatory increase in CD4+ cytotoxic T-cells. Immune responses involving CD8+T-cells are severely deficient, providing a model to assess the role of CD8+ cells and class I MHC in various experimental systems. Hemachromatosis has been noted in certain genetic backgrounds.

Development

The B2m^tm1Unc allele was generated in the laboratory of Dr. Beverly Koller and Dr. Oliver Smithies at The University of North Carolina at Chapel Hill. This targeted disruption used the 129P2/OlaHsd derived E14TG2a ES cell line. The C3H/HeJ strain was produced in the laboratory of Dr. Derry Roopenian at The Jackson Laboratory by backcrossing the B2m^tm1Unc allele from a segregating 129;B6 background 13 times to C3H/HeJ inbred mice.

Allele

Symbol: B2m^tm1Unc
Name: targeted mutation 1, University of North Carolina
MGI accession ID: MGI:1857133
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: B2m
Marker name: beta-2 microglobulin
Chromosome: 2
Strain of origin: 129P2/OlaHsd
Molecular note: Insertion of a neomycin-resistance gene into the second exon.