These B2m knock-out mice exhibit a severely deficient immune response involving CD8+ T-cells.
Read More +Genetic Background | Generation |
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000659 C3H/HeJ |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | B2m | beta-2 microglobulin |
Mice homozygous for the B2mtm1Unc targeted mutation have little if any MHC class I protein expression on the cell surface. There are few CD4- CD8+ cytotoxic T-cells and under some circumstances there is a compensatory increase in CD4+ cytotoxic T-cells. Immune responses involving CD8+T-cells are severely deficient, providing a model to assess the role of CD8+ cells and class I MHC in various experimental systems. Hemachromatosis has been noted in certain genetic backgrounds.
The B2mtm1Unc allele was generated in the laboratory of Dr. Beverly Koller and Dr. Oliver Smithies at The University of North Carolina at Chapel Hill. This targeted disruption used the 129P2/OlaHsd derived E14TG2a ES cell line. The C3H/HeJ strain was produced in the laboratory of Dr. Derry Roopenian at The Jackson Laboratory by backcrossing the B2mtm1Unc allele from a segregating 129;B6 background 13 times to C3H/HeJ inbred mice.
Allele Name | targeted mutation 1, University of North Carolina |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | b2mnull; beta2M-; beta2m0; beta2mnull; beta2mo; beta2mtm1Unc; beta2MKO; beta2-m-KO; I0; MHC-I- |
Gene Symbol and Name | B2m, beta-2 microglobulin |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 2 |
Molecular Note | Insertion of a neomycin-resistance gene into the second exon. |
Mutations Made By | Dr. Oliver Smithies, University of North Carolina at Chapel Hill |
When using the Β2M- mouse strain in a publication, please cite the originating article(s) and include JAX stock #004040 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Homozygous for B2m<tm1Unc>, 1 pair minimum |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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