These Z/AP reporter mice express a transgene containing a floxed Beta-geo and the human placental alkaline phosphatase gene with widespread expression of lacZ except in erythrocytes, chondrocytes and adipocytes.
Corrinne Lobe, Sunnybrook & Women's College HSC
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
These transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread with notable exceptions being erythrocytes, chondrocytes and adipocytes. Expression is observed throughout all embryonic and adult stages. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with alkaline phosphatase expression in tissues expressing Cre. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess cre excision activity at the individual cell level.
A transgenic construct containing the CMV enhancer/chicken beta-actin promoter, a loxP-flanked Bgeo reporter/SV40 polyadenylation signal segment, and the human placental alkaline phosphatase gene followed by a rabbit beta-globin polyadenylation sequence, was electroporated into 129X1/SvJ x 129S1/Sv-derived R1 embryonic stem (ES) cells. ES cells expressing lacZ were selected and utilized in ES cell/diploid embryo aggregation in conjunction with CD1 outbred embryos resulting in chimeric animals.
Expressed Gene | ALPP, alkaline phosphatase, placental, human |
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Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli |
Site of Expression | lacZ is widely expressed throughout embryonic and adult tissues with notable exceptions being erythrocytes, chondrocytes and adipocytes; when crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with alkaline phosphatase expression |
Allele Name | transgene insertion 1, Corrinne Lobe |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | Tg(ACTB-Bgeo/ALPP)1Lbe; Tg(CAG-Bgeo/ALPP)1Lbe; Z/AP; ZAP |
Gene Symbol and Name | Tg(CAG-Bgeo/ALPP)1Lbe, transgene insertion 1, Corrinne Lobe |
Gene Synonym(s) | |
Promoter | ACTB, actin, beta, chicken |
Expressed Gene | ALPP, alkaline phosphatase, placental, human |
Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli |
Site of Expression | lacZ is widely expressed throughout embryonic and adult tissues with notable exceptions being erythrocytes, chondrocytes and adipocytes; when crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with alkaline phosphatase expression |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | UN |
General Note | Cre excision of lacZ and the neomycin resistance gene results in expression of the ALPP reporter. |
Molecular Note | The transgene contains the CMV enhancer/chicken beta-actin promoter, a floxed Beta-geo reporter/SV40 polyadenylation signal segment, and the human placental alkaline phosphatase gene followed by a rabbit Hbb polyadenylation sequence. In transgenic mice expression of lacZ is widespread, with notable exceptions being erythrocytes, chondrocytes and adipocytes. This expression is observed throughout all embryonic and adult stages. |
Mutations Made By | Corrinne Lobe, Sunnybrook & Women's College HSC |
This strain arose on the 129X1/SvJ x 129S1/Sv (R1 ES cell line) background and was maintained by the donating investigator as a hemizygote by crosses to wildtype outbred CD1 mice. It is unknown if homozygotes are viable. Coat color expected from breeding:Agouti
When using the Z/AP reporter mouse strain in a publication, please cite the originating article(s) and include JAX stock #003919 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(CAG-Bgeo/ALPP)1Lbe |
Frozen Mouse Embryo | STOCK Tg(CAG-Bgeo/ALPP)1Lbe/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(CAG-Bgeo/ALPP)1Lbe/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(CAG-Bgeo/ALPP)1Lbe/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(CAG-Bgeo/ALPP)1Lbe/J Frozen Embryos | $3373.50 |
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