STOCK Tg(CAG-Bgeo/ALPP)1Lbe/J

Stock number: 003919
Product name: Z/AP reporter
Research areas: Research Tools

Description

These Z/AP reporter mice express a transgene containing a floxed Beta-geo and the human placental alkaline phosphatase gene with widespread expression of lacZ except in erythrocytes, chondrocytes and adipocytes.

Detailed description

These transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread with notable exceptions being erythrocytes, chondrocytes and adipocytes. Expression is observed throughout all embryonic and adult stages. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with alkaline phosphatase expression in tissues expressing Cre. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess cre excision activity at the individual cell level.

Development

A transgenic construct containing the CMV enhancer/chicken beta-actin promoter, a loxP-flanked Bgeo reporter/SV40 polyadenylation signal segment, and the human placental alkaline phosphatase gene followed by a rabbit beta-globin polyadenylation sequence, was electroporated into 129X1/SvJ x 129S1/Sv-derived R1 embryonic stem (ES) cells. ES cells expressing lacZ were selected and utilized in ES cell/diploid embryo aggregation in conjunction with CD1 outbred embryos resulting in chimeric animals.

Allele

Symbol: Tg(CAG-Bgeo/ALPP)1Lbe
Name: transgene insertion 1, Corrinne Lobe
MGI accession ID: MGI:3505556
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(CAG-Bgeo/ALPP)1Lbe
Marker name: transgene insertion 1, Corrinne Lobe
Chromosome: UN
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Expressed genes: Bgeo,fusion of beta-galactosidase and neomycin phosphotransferase genes,E. coli; ALPP,alkaline phosphatase, placental,human
Site of expression: lacZ is widely expressed throughout embryonic and adult tissues with notable exceptions being erythrocytes, chondrocytes and adipocytes; when crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with alkaline phosphatase expression
Molecular note: The transgene contains the CMV enhancer/chicken beta-actin promoter, a floxed Beta-geo reporter/SV40 polyadenylation signal segment, and the human placental alkaline phosphatase gene followed by a rabbit Hbb polyadenylation sequence. In transgenic mice expression of lacZ is widespread, with notable exceptions being erythrocytes, chondrocytes and adipocytes. This expression is observed throughout all embryonic and adult stages.
General note: Cre excision of lacZ and the neomycin resistance gene results in expression of the ALPP reporter.