B6.129S7-Sod1^tm1Leb/DnJ

Stock number: 003881
Product name: Sod1 KO
Research areas: Metabolism Research, Neurobiology Research

Description

Homozygous Sod1^tm1Leb knock-out mice are characterized by abnormal retinal and cochlear ganglions, and they have age-related defects in hearing, vasculature, and muscle. These mice may be useful in studying retinal dysfunction, autoimmunity, female reproductive development/function, glaucoma, hearing loss, Alzheimer's Disease, familial amyotrophic lateral sclerosis (ALS), and other age-related disorders.

Detailed description

The Sod1^tm1Leb (formerly SOD1^m1BCM) knock-out allele disrupts exons 1 and 2 of the Cu,Zn-superoxide dismutase-1 gene (Sod1). This knock-out allele is the same as carried by B6;129S-Sod1^tm1Leb/J (Stock No. 002972) but is fully congenic in the present strain having been backcrossed to C57BL/6JEi for 13 generations. In the original strain no protein expression from the targeted locus is detected in brain tissues. Heterozygous mice are viable and fertile. While homozygous males reproduce normally, homozygous females are infertile. Homozygous mice have abnormalities of the retinal ganglion, cochlear ganglion, and female reproductive development/function. Homozygous mice also exhibit age-related deafness, accelerated vascular aging and age-related skeletal muscle defects. These mutant mice may be useful in studying retinal dysfunction, autoimmunity, glaucoma, hearing loss, Alzheimer's Disease, familial amyotrophic lateral sclerosis (ALS), and other age-related disorders.

Development

This strain was generated in the Laboratory of Muriel Davisson by backcrossing the Sod1^tm1Leb mutation from the segregating B6;129S7 background onto the C57BL/6JEi strain for 13 generations. The backcrossing was begun in 1998. In 2004 embryos were generated for cryopreservation via in vitro fertilization using C57BL/6J females and heterozygous males at generation N13.

Allele

Symbol: Sod1^tm1Leb
Name: targeted mutation 1, Russell M Lebovitz
MGI accession ID: MGI:1857873
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Sod1
Marker name: superoxide dismutase 1, soluble
Chromosome: 16
Strain of origin: 129S7/SvEvBrd-Hprt^b-m2
Molecular note: Replacement of exons 1 and 2 with a PGK-hprt expression cassette. Protein was not detected in brain of homozygous mutant mice.