Overview

This strain carries the brachyury allele (Tand the spontaneous Itpr3^tf mutation, derived from the now extinct TF/Le strain, and is characterized by tail defects, hair loss and regrowth.

Genetic overview

Genetic Background	Generation

a

Allele Type	Gene Symbol	Gene Name
Spontaneous	a	nonagouti

T

Allele Type	Gene Symbol	Gene Name
Spontaneous	T	brachyury, T-box transcription factor T

Allele Type	Gene Symbol	Gene Name
Spontaneous	Itpr3	inositol 1,4,5-triphosphate receptor 3

Research Applications

Dermatology Research
Developmental Biology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

This strain carries the brachyury allele (Tand the spontaneous Itpr3^tf mutation, derived from the now extinct TF/Le strain, and is characterized by tail defects, hair loss and regrowth.

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Additional Information

Considerations for Choosing Controls

Genetics

a

Allele Symbol: a

Allele Name	nonagouti
Allele Type	Spontaneous
Allele Synonym(s)
Gene Symbol and Name	a, nonagouti
Gene Synonym(s)
Strain of Origin	old mutant of the mouse fancy
Chromosome	2
General Note	Insertion of the LV30 retrotransposon without the beta4 retrovirus sequence does not cause the nonagouti phenotype. J:278039
Molecular Note	Characterization of this allele shows an insertion of DNA comprised of a 5.5kb virus-like element, VL30, into the first intron of the agouti gene. The VL30 element itself contains an additional 5.5 kb sequence, flanked by 526 bp of direct repeats (beta4 retroviral sequence). The host integration site is the same as for a^t-2Gso and A^w-38J and includes a duplication of four nucleotides of host DNA and a deletion of 2 bp from the end of each repeat. Northern analysis of mRNA from skin of homozygotes shows a smaller agouti message and levels 8 fold lower than found in wild-type.

T

Allele Symbol: T

Allele Name	brachyury
Allele Type	Spontaneous
Allele Synonym(s)	T1
Gene Symbol and Name	T, brachyury, T-box transcription factor T
Gene Synonym(s)
Strain of Origin	Laboratory stock
Chromosome	17
Molecular Note	Pulsed-field gel electrophoresis revealed an altered restriction fragment size consistent with a deletion of 160-200kb.

Itpr3^tf

Allele Symbol: Itpr3^tf

Allele Name	tufted
Allele Type	Spontaneous
Allele Synonym(s)	tf
Gene Symbol and Name	Itpr3, inositol 1,4,5-triphosphate receptor 3
Gene Synonym(s)
Strain of Origin	BTBR
Chromosome	17
General Note	This allele was recovered from a Harwell testing stock carrying multiple recessive markers in an undefined background. (J:273)
Molecular Note	Complementation mapping was used to demonstrate that this spontaneous mutation was an allele of Itpr3. Sequencing revealed a 12 bp deletion in Exon23 (Chr17: 27238069, Build 38.1) which codes for amino acids 983-986. This mutation arose early in the history of the BTBR strain (in or soon after 1956) and is not found in 18 other strains (129P2/OlaHsd, 129S1/SvImJ, 129S5/SvEvBrd, A/J, AKR/J, BALB/cJ, C3H/HeJ, C57BL/6NJ, CAST/EiJ, CBA/J, DBA/2J, FVB/NJ, LP/J, NOD/ShiLtJ, NZO/HlLtJ, PWK/PhJ, SPRET/EiJ and WSB/EiJ)

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Itpr3^tf related

Dermatology Research
- Skin and Hair Texture Defects

Genotype: T related

Developmental Biology Research
- Skeletal Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Itpr3^tf/Itpr3^tf
involves: BTBR

behavior/neurological phenotype

abnormal liquid preference
- Normal - however, mice exhibit normal response to capsaicin
- in a 48 h two-bottle choice test, mice exhibit higher preference for calcium chloride, calcium lactate, denatorium, quinine hydrochloride and caffeine compared with NZW mice
- in a 48 h two-bottle choice test, mice exhibit lower preference for sodium chloride, inosine monophosphate, trisodium pyrophosphate, saccharin, sucrose and hydrochloride compared with NZW mice
- in a 96 h two-bottle choice tests, mice exhibit higher preference for calcium chloride and calcium lactate compared with NZW mice
- in a 96 h two-bottle choice tests, mice exhibit lower preference for saccharin and citric acid compared with NZW mice

increased fluid intake
- compared with NZW mice

low sweet preference
- in a 48 h two-bottle choice test, mice exhibit lower preference for saccharin and sucrose compared with NZW mice
- in a 96 h two-bottle choice tests, mice exhibit lower preference for saccharin compared with NZW mice

integument phenotype

abnormal coat/ hair morphology
- alternating waves of hair loss and growth are repeated throughout the life of the mouse
- detectable about 4 weeks of age

abnormal hair cycle
- a few days after ventral hair loss a similar dorsal wave of hair loss begins at the face, not including whiskers
- a normal wave of hair growth follows the initial loss pattern
- a wave of hair loss begins ventrally under the jaw and spreads posteriorly

abnormal hair growth

sparse hair
- waves of hair loss and growth create a sparse hair appearance anterior to posterior, ventral and dorsal

taste/olfaction phenotype

abnormal gustatory system physiology
- Normal - however, mice exhibit normal response to capsaicin
- in a 48 h two-bottle choice test, mice exhibit higher preference for calcium chloride, calcium lactate, denatorium, quinine hydrochloride and caffeine compared with NZW mice
- in a 48 h two-bottle choice test, mice exhibit lower preference for sodium chloride, inosine monophosphate, trisodium pyrophosphate, saccharin, sucrose and hydrochloride compared with NZW mice
- in a 96 h two-bottle choice tests, mice exhibit higher preference for calcium chloride and calcium lactate compared with NZW mice
- in a 96 h two-bottle choice tests, mice exhibit lower preference for saccharin and citric acid compared with NZW mice

growth/size/body region phenotype

increased body weight
- male, but not female, mice exhibit a slight increase in body weight compared with NZW mice

References

Additional References

Flood WD; Ruvinsky A. 2001. Alternative splicing and expressivity of the AxinFu allele in mice. Heredity 87(Pt 2):146-52PubMed: 11703504 MGI: J:72363
Ruvinsky A; Flood WD; Zhang T; Costantini F. 2000. Unusual inheritance of the AxinFu mutation in mice is associated with widespread rearrangements in the proximal region of chromosome 17. Genet Res 76(2):135-47PubMed: 11132407 MGI: J:68896

Additional - a related

Additional - Itpr3^tf related

Additional - T related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Appearance
- black, tail defects, tufted
  Related Genotype: a/a T tf/+ tf
  
  black, tufted
  Related Genotype: a/a, + tf/+ tf
Citation
When using the B10;TFLe-a/a T Itpr3^tf/+ Itpr3^tf/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #003879 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Homozygous for tf, Heterozygous or Wild-type for T

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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