These Mif knock-out mice exhibit resistance to lethal effects of endotoxemia in response to intraperitoneally injected LPS.
John R. David, Harvard School of Public Health
These mice have a neo cassette disrupting exons 2-3 of the macrophage migration inhibitory factor (glycosylation-inhibiting factor) (MIf) gene, abolishing gene expression. MIF is a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function. Mice that are homozygous null for the Mif gene are viable and fertile. No Mif gene product (mRNA or protein) is detected. While null mice exhibit signs of endotoxemia in response to intraperitoneally injected LPS (25 mg/kg), they are resistant to its lethal effects. Plasma levels of tumor necrosis factor alpha assayed after LPS administration is half that observed in wildtype mice.
A targeting vector containing a neomycin resistance gene and a herpes simplex virus thymidine kinase gene was used to disrupt exons 2-3 of the macrophage migration inhibitory factor (glycosylation-inhibiting factor) (MIf) gene. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells and correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were backcrossed to BALB/cAnNTac mice for at least 6 generations.
|Allele Name||targeted mutation 1, John R David|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||MIF-; MIF-KO|
|Gene Symbol and Name||Mif, macrophage migration inhibitory factor (glycosylation-inhibiting factor)|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A neomycin resistance cassette replaced a genomic fragment containing part of exon 2 and exon 3. Northern blot analysis on RNA derived from liver of LPS-treated homozygous mice demonstrated that no detectable transcript was produced form this allele. ELISA assays on serum of LPS-treated animals confirmed that no functional protein was expressed from this allele.|
|Mutations Made By|| |
John David, Harvard School of Public Health
When maintaining a live colony, homozygous mice may be bred together.
When using the MIF- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003830 in your Materials and Methods section.