Mice homozygous for the Cd1tm1Gru knock-out mutation are deficient in both the Cd1d1 and Cd1d2 genes (CD1.1, CD1.2). Homozygous mutant mice lack the normal natural killer cell -like T cell subset.
Dr. Michael J. Grusby, Harvard Medical School
Mice homozygous for the Cd1tm1Gru targeted mutation are deficient in both the Cd1d1 and Cd1d2 genes (CD1.1, CD1.2). Homozygous mutant mice lack the normal natural killer cell -like T cell subset. However, type 2 T helper cell (TH2) response is normal, demonstrating that this T cell subset is not required for TH2 response, as previously believed.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
The targeting vector used deleted most of the Cd1d1 and Cd1d2 coding regions. The intergenic sequence was maintined.
|Allele Name||targeted mutation 1, Michael Grusby|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||CD1-; CD1d KO; Cd1d-; Cd1d1/Cd1d2tm1Gru; CD1tm1Gru|
|Gene Symbol and Name||Cd1, CD1 antigen complex|
|Strain of Origin||129S2/SvPas|
|Molecular Note||Both the Cd1d1 gene and the adjacent Cd1d2 gene were mutated in this allele. The coding sequence of Cd1d1 was replaced with a neomycin selection cassette and the coding sequence of Cd1d2 was deleted; however, the intergenic sequence was retained. Flow cytometric analysis on thymocytes derived from homozygous mice confirmed that no stable encoded protein was expressed on the cell surface.|
|Mutations Made By|| |
Dr. Michael Grusby, Harvard Medical School
This strain originated on a C;129S background and has been backcrossed to BALB/cJ (N11). It is maintained by homozygous sibling matings. Coat color expected from breeding:Albino
When using the CD1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003814 in your Materials and Methods section.