C.129S2-Cd1^tm1Gru/J

Stock number: 003814
Product name: CD1^-
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

Mice homozygous for the Cd1^tm1Gru knock-out mutation are deficient in both the Cd1d1 and Cd1d2 genes (CD1.1, CD1.2). Homozygous mutant mice lack the normal natural killer cell -like T cell subset.

Detailed description

Mice homozygous for the Cd1^tm1Gru targeted mutation are deficient in both the Cd1d1 and Cd1d2 genes (CD1.1, CD1.2). Homozygous mutant mice lack the normal natural killer cell -like T cell subset. However, type 2 T helper cell (TH2) response is normal, demonstrating that this T cell subset is not required for TH2 response, as previously believed.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

The targeting vector used deleted most of the Cd1d1 and Cd1d2 coding regions. The intergenic sequence was maintined.

Allele

Symbol: Cd1^tm1Gru
Name: targeted mutation 1, Michael Grusby
MGI accession ID: MGI:1857482
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Cd1
Marker name: CD1 antigen complex
Chromosome: 3
Strain of origin: 129S2/SvPas
Molecular note: Both the Cd1d1 gene and the adjacent Cd1d2 gene were mutated in this allele. The coding sequence of Cd1d1 was replaced with a neomycin selection cassette and the coding sequence of Cd1d2 was deleted; however, the intergenic sequence was retained. Flow cytometric analysis on thymocytes derived from homozygous mice confirmed that no stable encoded protein was expressed on the cell surface.