B6.129S2(Cg)-Prl^tm1Hmn/J

Stock number: 003808
Product name: PRL-
Research areas: Developmental Biology Research, Endocrine Deficiency Research, Reproductive Biology Research

Description

Homozygous Prl knock-out females are completely infertile and exhibit an irregular estrous cycle and abnormal mammary gland development.

Detailed description

Mice that are homozygous for the null Prl allele are viable, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous null females have an irregular estrous cycle and are completely infertile. Homozygous null males and heterozygous females exhibit no fertility problems. The targeted insertion of a neomycin resistance gene into exon 4 results in a truncated Prl transcript that produces an 11 kDa prolactin protein that lacks any detectable bioactivity. Mammary gland development is marked by an absence of terminal or lateral lobulation. The disruption of the prolactin gene appears to have no effects on the hematopoietic system.

Development

A targeting vector containing a neomycin resistance gene driven by the mouse phosphoglycerate kinase promoter and a herpes simplex virus thymidine kinase was used to disrupt Prl exon 4 which encodes the second alpha helix of the prolactin protein. The construct was transfected into D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and chimeric animals generated.The chimeric mouse was mated to Black Swiss outbred mice and then backcrossed to C57BL/6J for at least eight generations.

Allele

Symbol: Prl^tm1Hmn
Name: targeted mutation 1, Nelson D Horseman
MGI accession ID: MGI:1934021
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: PRL-
Marker symbol: Prl
Marker name: prolactin
Marker synonyms: Gha1; Prl1a1; PRLB; PRLSD1; Prol; RATPRLSD1; RNPROL
Chromosome: 13
Strain of origin: 129S2/SvPas
Molecular note: A PGK neomycin cassette was inserted into exon 4. This sequence encodes the second alpha helix of the protein. RT-PCR analysis confirmed a lack of wild-type transcript in homozygous mutant mice. Western blotting of pituitary gland extracts revealed the presence of a unique immunoreactive ~10-kDa truncated protein product in homozygous mutant mice. Disrupted prolactin synthesis was confirmed by biological assays.