B6.Cg-Tg(Lck-cre)548Jxm/J

Stock number: 003802
Research areas: Research Tools

Description

This transgenic strain expresses Cre under the control of the Lck (lymphocyte protein tyrosine kinase) promoter, enabling thymocyte-specific excision of loxP-flanked sequences of interest. The Lck gene is primarily expressed by T lymphocytes and it plays a key role in the selection and maturation of developing T-cells in the thymus and in the activity of mature T-cells.

Detailed description

This transgenic strain expresses Cre under the control of the Lck (lymphocyte protein tyrosine kinase) promoter, enabling thymocyte-specific excision of loxP-flanked sequences of interest. Homozygous mice are viable and have no major defects.

When crossed with floxed Galnt13 mice (see Stock No. 006896), excision occurs at high efficiency in thymocytes. Splenic CD4⁺ T cells show excision at somewhat lower rates (66-83%).

The Lck gene is primarily expressed by T lymphocytes where it phosphorylates tyrosine residues of proteins involved with intracellular signaling pathways. Associating with CD4 and CD8, LCK is part of a cascade of responses to antigen that control the proliferation and activation of T cells. It plays a key role in the selection and maturation of developing T-cells in the thymus and in the activity of mature T-cells.

View cre expression characterization.

Development

A transgenic construct containing a Cre recombinase gene with a human growth hormone polyadenylation signal under the control of the mouse Lck promoter was used to create this mutation. The construct was injected either into B6D2F1 or B6CBAF2 zygotes to obtain founder animals.

In Goodwin et al. 2019 Genome Res 29:494, it was discovered that Stock No. 003802 has more than 20 transgene copies integrated on chromosome 11 [41,490,714]. This study reports that no gene/locus was disrupted.

Allele

Symbol: Tg(Lck-cre)548Jxm
Name: transgene insertion 548, Jamey Marth
MGI accession ID: MGI:2176199
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Lck-cre)548Jxm
Marker name: transgene insertion 548, Jamey Marth
Chromosome: 11
Strain of origin: (C57BL/6 x CBA)F2
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: thymocytes
Molecular note: This transgene expresses Cre recombinase with a human growth hormone polyadenylation signal under the control of the mouse lck promoter, active in the T cell lineage. Transgene insertion occurred on Chr11, causing a 1 bp duplication.
General note: Homozygous transgenic mice are viable and have no major defects.