This transgenic strain expresses Cre under the control of the Lck (lymphocyte protein tyrosine kinase) promoter, enabling thymocyte-specific excision of loxP-flanked sequences of interest. The Lck gene is primarily expressed by T lymphocytes and it plays a key role in the selection and maturation of developing T-cells in the thymus and in the activity of mature T-cells.
Jamey D Marth, Burnham Inst at Univ Calif Santa Barbara
Genetic Background | Generation |
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N10+pN3
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Allele Type |
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Transgenic (Recombinase-expressing) |
Starting at:
$255.00 Domestic price for female 4-week |
333.51 Domestic price for breeder pair |
This transgenic strain expresses Cre under the control of the Lck (lymphocyte protein tyrosine kinase) promoter, enabling thymocyte-specific excision of loxP-flanked sequences of interest. Homozygous mice are viable and have no major defects.
When crossed with floxed Galnt13 mice (see Stock No. 006896), excision occurs at high efficiency in thymocytes. Splenic CD4+ T cells show excision at somewhat lower rates (66-83%).
The Lck gene is primarily expressed by T lymphocytes where it phosphorylates tyrosine residues of proteins involved with intracellular signaling pathways. Associating with CD4 and CD8, LCK is part of a cascade of responses to antigen that control the proliferation and activation of T cells. It plays a key role in the selection and maturation of developing T-cells in the thymus and in the activity of mature T-cells.
View cre expression characterization.
A transgenic construct containing a Cre recombinase gene with a human growth hormone polyadenylation signal under the control of the mouse Lck promoter was used to create this mutation. The construct was injected either into B6D2F1 or B6CBAF2 zygotes to obtain founder animals.
In Goodwin et al. 2019 Genome Res 29:494, it was discovered that Stock No. 003802 has more than 20 transgene copies integrated on chromosome 11 [41,490,714]. This study reports that no gene/locus was disrupted.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | thymocytes |
Allele Name | transgene insertion 548, Jamey Marth |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Lck-cre; pLck-cre; Tg(Lck-cre); TgN(Lck-cre)548Jxm |
Gene Symbol and Name | Tg(Lck-cre)548Jxm, transgene insertion 548, Jamey Marth |
Gene Synonym(s) | |
Promoter | Lck, lymphocyte protein tyrosine kinase, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | thymocytes |
Strain of Origin | (C57BL/6 x CBA)F2 |
Chromosome | 11 |
General Note | Homozygous transgenic mice are viable and have no major defects. |
Molecular Note | This transgene expresses Cre recombinase with a human growth hormone polyadenylation signal under the control of the mouse lck promoter, active in the T cell lineage. Transgene insertion occurred on Chr11, causing a 1 bp duplication. |
Mutations Made By | Jamey Marth, Burnham Inst at Univ Calif Santa Barbara |
When using the B6.Cg-Tg(Lck-cre)548Jxm/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #003802 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous for Tg(Lck-cre)548Jxm |
Frozen Mouse Embryo | B6.Cg-Tg(Lck-cre)548Jxm/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Lck-cre)548Jxm/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Lck-cre)548Jxm/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Lck-cre)548Jxm/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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