B6;SJL-Tg(ACTFLPe)9205Dym/J

Stock number: 003800
Product name: ACTB:FLPe B6;SJL
Research areas: Research Tools

Description

This transgenic strain expresses a FLP1 recombinase gene under the direction of the human ACTB promoter. Recombinase activity is detected in a wide variety of tissues (spinal cord, heart, gonad, adrenal) as early as embryonic day 10.5. This deleter strain is a suitable alternative to, and complement with the Cre-loxP system for in vivo genetic engineering.

Detailed description

This transgenic strain expresses a variant of the Saccharomyces cerevisiae FLP1 recombinase gene under the direction of the human ACTB promoter. The FLPe recombinase variant exhibits enhanced thermostability with recombination activity being four-fold and ten-fold that of wildtype FLP at 37C and 40C, respectively. Mice that are hemizygous for the transgenic allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity is detected in a wide variety of tissues (spinal cord, heart, gonad, adrenal) as early as embryonic day 10.5. This deleter strain is a suitable alternative to, and complement with the Cre-loxP system for in vivo genetic engineering.

Development

A transgenic construct containing a FLP1 recombinase variant controlled by the human ACTB promoter (3 kb flanking sequence, 78-bp 5' untranslated region, 832-bp of exon 1) was injected into fertilized eggs from B6SJLF2 mice. Founder animals were obtained and crossed with C57BL/6J mice. This strain was backcrossed to C57BL/6J at least three times prior to sending to The Jackson Laboratory in 2000. Upon arrival, the colony was maintained by breeding hemizygous mice with wildtype (noncarrier) animals for several generations. In 2008, hemizygous mice were bred together to make the colony homozygous. In 2012, the colony was bred once with B6SJLF1/J (Stock No. 100012), and then maintained by breeding homozygous mice together.

Allele

Symbol: Tg(ACTFLPe)9205Dym
Name: transgene insertion 9205, Susan Dymecki
MGI accession ID: MGI:2448985
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(ACTFLPe)9205Dym
Marker name: transgene insertion 9205, Susan Dymecki
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F2
Expressed genes: FLP,FLP recombinase,Saccharomyces cerevisiae
Site of expression: expression of the enhanced FLP1 recombinase variant (FLPe) is detected in a wide variety of tissues (spinal cord, heart, gonad, adrenal) as early as embryonic day 10.5
Molecular note: Sequence encoding FLP1 was adjoined to human ACTB promoter sequence, including 3 kb of promoter sequence and an 0.8 kb portion of exon 1. The promoter region drives expression in the germline. Recombinase activity is detected in a wide variety of tissues (spinal cord, heart, gonad, adrenal) as early as embryonic day 10.5.
General note: The FLPe recombinase variant exhibits enhanced thermostability with recombination activity being 4X and 10X that of wildtype FLP at 37°C and 40°C, respectively. Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities.