B6.Cg-Tg(Eno2tTA)5021Nes/J

Stock number: 003767
Product name: NSE-tTA line B
Research areas: Neurobiology Research, Research Tools

Description

These NSE-tTA mice express a transgene containing the tetracycline-controlled activator protein (tTA) under the control of a rat neuron-specific enolase (Eno2) promoter, and selectively expresses tTA at high levels in the striatum and cerebellum.

Detailed description

These transgenic mice express the tetracycline-controlled activator protein (tTA) under the control of a rat neuron-specific enolase (Eno2) promoter. Although the Eno2 promoter has been shown to direct high levels of expression in brain tissue in general, this strain selectively expresses tTA at high levels in the striatum and cerebellum. When B6.Cg-Tg(Eno2tTA)5021Nes/J transgenic mice are mated to a second transgenic strain carrying a gene of interest under the regulation of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene in the bitransgenic offspring may be induced in the tTA-expressing tissues by the withdrawal of the tetracycline analog, doxycycline (dox). For example, when these transgenic mice are bred with Stock No. 003762, a transgenic strain that expresses a Fosb variant under the direction of a TRE, the resulting bitransgenic offspring can be induced to express high levels of the Fosb variant in the striatum and cerebellum by withdrawing dox.

Development

A transgenic construct containing a modified tTa cDNA under the control of an Eno2 (Enolase 2, gamma, neuronal) promoter, an SV40 intron and a poly A signal sequence, was injected into the pronuclei of oocytes from B6;SJL mice. Founder mice (line B in the primary reference) were backcrossed to C57BL/6J mice.

Allele

Symbol: Tg(Eno2tTA)5021Nes
Name: transgene insertion 5021, Eric Nestler
MGI accession ID: MGI:3053186
Generation method: Transgenic
Attributes: Transactivator
Synonyms: line B; NSE-tTA
Marker symbol: Tg(Eno2tTA)5021Nes
Marker name: transgene insertion 5021, Eric Nestler
Marker synonyms: line B; NSE-tTA
Chromosome: UN
Strain of origin: (SJL x C57BL/6)F1
Expressed genes: tTA,tetracycline-controlled transactivator,E. coli
Site of expression: Expresses tTA at high levels in the striatum and cerebellum.
Molecular note: This transgene consists of a 1.8kb fragment of the rat Eno2 promoter, an SV40 intron, a poly A signal sequence, and a modified tetracycline transactivator cDNA.
General note: B6.Cg-TgN(Eno2tTA)5021Nes mice selectively express tTA at high levels in the striatum and cerebellum. When these transgenic mice are mated to a second transgenic strain which carries a gene of interest under the regulation of a tetracycline-responsive promoter element (TRE), expression of the target gene in the bitransgenic offspring may be induced in those tissues where tTA is expressed by the withdrawal of the tetracycline analog, doxycycline (dox).