B6.Cg-Tg(tetFosb)4468Nes/J

Stock number: 003762
Product name: TetOp-ΔFosB line 11
Research areas: Neurobiology Research, Research Tools

Description

These TetOp-ΔFosB mice express a truncated variant of the Fosb transcription factor with transcription directed by a tetracycline-responsive promoter element (TRE; tetO). The expressed variant is induced in the brain upon repeated exposure to commonly abused drugs.

Detailed description

Mice homozygous for the Fosb transgene are viable and fertile. These mice express a truncated variant of the Fosb transcription factor. The expressed variant is a highly stable isoform found to be induced in the brain (nucleus accumbens) with repeated exposure to commonly abused drugs, such as amphetamine, morphine, nicotine, and phencyclidine. Transcription is directed by a tetracycline-responsive promoter element (TRE; tetO). When hemizygotes are bred with another transgenic mouse expressing tetracycline-controlled transactivator protein (tTA or rtTA) to create a bitransgenic animal, Fosb expression can be regulated with the tetracycline analog, doxycycline. This transgenic strain can be crossed with strains 003763 or 003767 to produce bitransgenic mice that can be induced to express high levels of the Fosb variant in brain tissue by withdrawing doxycycline.

Development

A transgenic construct containing a Fosb cDNA under the control of a tetracycline-regulated promoter (TRE; tetO), an SV40 intron and a poly A signal sequence, was injected into the pronuclei of oocytes from B6 x SJL hybrid mice. Founder mice were mated to ICR mice and then backcrossed to C57BL/6J mice.

Allele

Symbol: Tg(tetFosb)4468Nes
Name: transgene insertion 4468, Eric Nestler
MGI accession ID: MGI:3053111
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(tetFosb)4468Nes
Marker name: transgene insertion 4468, Eric Nestler
Chromosome: UN
Strain of origin: (SJL x C57BL/6)F1
Expressed genes: Fosb,FBJ osteosarcoma oncogene B,mouse, laboratory
Site of expression: When mated to a strain expressing tTA, Fosb expression can be negatively regulated with the tetracycline analog, doxycycline.
Molecular note: The transgene contains a Fosb cDNA under the control of a tetracycline-regulated promoter, an SV40 intron, and a poly A signal sequence.
General note: Homozygous transgenic mice are viable and fertile. These mice express a truncated variant of the Fosb transcription factor. The expressed variant is a highly stable isoform found to be induced in the brain (nucleus accumbens) with repeated exposure to commonly abused drugs, such as amphetamine, morphine, nicotine, and phencyclidine. Transcription is directed by a tetracycline-responsive promoter element (TRE). In bitransgenic mice produced by breeding a mouse carrying this transgene with a transgenic mouse expressing tetracycline-controlled transactivator protein (tTA), Fosb expression can be negatively regulated with the tetracycline analog, doxycycline.