These TetOp-ΔFosB mice express a truncated variant of the Fosb transcription factor with transcription directed by a tetracycline-responsive promoter element (TRE; tetO). The expressed variant is induced in the brain upon repeated exposure to commonly abused drugs.
Dr. Eric Nestler, UT Southwestern Medical Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Mice homozygous for the Fosb transgene are viable and fertile. These mice express a truncated variant of the Fosb transcription factor. The expressed variant is a highly stable isoform found to be induced in the brain (nucleus accumbens) with repeated exposure to commonly abused drugs, such as amphetamine, morphine, nicotine, and phencyclidine. Transcription is directed by a tetracycline-responsive promoter element (TRE; tetO). When hemizygotes are bred with another transgenic mouse expressing tetracycline-controlled transactivator protein (tTA or rtTA) to create a bitransgenic animal, Fosb expression can be regulated with the tetracycline analog, doxycycline. This transgenic strain can be crossed with strains 003763 or 003767 to produce bitransgenic mice that can be induced to express high levels of the Fosb variant in brain tissue by withdrawing doxycycline.
A transgenic construct containing a Fosb cDNA under the control of a tetracycline-regulated promoter (TRE; tetO), an SV40 intron and a poly A signal sequence, was injected into the pronuclei of oocytes from B6 x SJL hybrid mice. Founder mice were mated to ICR mice and then backcrossed to C57BL/6J mice.
Expressed Gene | Fosb, FBJ osteosarcoma oncogene B, mouse, laboratory |
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Site of Expression | When mated to a strain expressing tTA, Fosb expression can be negatively regulated with the tetracycline analog, doxycycline. |
Allele Name | transgene insertion 4468, Eric Nestler |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | TetOp-*FosB line 11; TetOp-deltaFosB line 11 |
Gene Symbol and Name | Tg(tetFosb)4468Nes, transgene insertion 4468, Eric Nestler |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | Fosb, FBJ osteosarcoma oncogene B, mouse, laboratory |
Site of Expression | When mated to a strain expressing tTA, Fosb expression can be negatively regulated with the tetracycline analog, doxycycline. |
Strain of Origin | (SJL x C57BL/6)F1 |
Chromosome | UN |
General Note | Homozygous transgenic mice are viable and fertile. These mice express a truncated variant of the Fosb transcription factor. The expressed variant is a highly stable isoform found to be induced in the brain (nucleus accumbens) with repeated exposure to commonly abused drugs, such as amphetamine, morphine, nicotine, and phencyclidine. Transcription is directed by a tetracycline-responsive promoter element (TRE). In bitransgenic mice produced by breeding a mouse carrying this transgene with a transgenic mouse expressing tetracycline-controlled transactivator protein (tTA), Fosb expression can be negatively regulated with the tetracycline analog, doxycycline. |
Molecular Note | The transgene contains a Fosb cDNA under the control of a tetracycline-regulated promoter, an SV40 intron, and a poly A signal sequence. |
Mutations Made By | Dr. Eric Nestler, UT Southwestern Medical Center |
This strain originated on a B6;SJL background. Founder mice were crossed to ICR before being backcrossed to C57BL/6J (N6 7/20/00)
When using the TetOp-ΔFosB line 11 mouse strain in a publication, please cite the originating article(s) and include JAX stock #003762 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non-carrier for Tg(tetFosb)4468Nes |
Frozen Mouse Embryo | B6.Cg-Tg(tetFosb)4468Nes/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(tetFosb)4468Nes/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(tetFosb)4468Nes/J | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(tetFosb)4468Nes/J | $3373.50 |
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