These mice harbor a recessive, lethal gene trap mutation, exhibiting failed neural tube closure resulting in exencephaly, acrania, and facial clefting by day E14.5.
Read More +Genetic Background | Generation |
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000664 C57BL/6J |
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Allele Type | Gene Symbol | Gene Name |
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Gene trapped (Reporter, Null/Knockout) | Shroom3 | shroom family member 3 |
Mice that are homozygous for this mutation survive to term or die very shortly after birth. The gene trap insertion appears to have occurred between the translational start sites of the long and short forms in the 5' portion of the endogenous gene. No gene product (protein or mRNA) is detected in homozygous embryos. Mutant embryos can be distinguished by E9.25 as the lateral edges of the cranial neural folds exhibit a wavy appearance and fail to converge at the dorsal midline. As the embryo develops, the neural folds continue to enlarge and develop away from the dorsal midline, presenting a mushroom-like appearance. By day E14.5, failed neural tube closure results in exencephaly, acrania, and facial clefting. Some mutants exhibit defects in ventral closure resulting in herniation of the intestine and liver. Not all aspects of the phenotype are fully penetrant. Hemizygous mice express a B-galactosidase under the control of the endogenous promoter, with expression variously observed in the neural tube, forebrain, somites, ventral body wall, heart, gut and skeletal muscle.
A retroviral vector ROSA-BgalCre containing a gene trap cassette was introduced into AK7 embryonic stem (ES) cells. The components of ROSA-BgalCre include an adenovirus splice acceptor, a bifunctional gene fusion between B-galactosidase and Cre recombinase, and the MC1 polyadenylation sequence. ES cells containing gene trap insertions were used to generate chimeric mice. Resulting chimeric male animals were backcrossed to wildtype C57BL/6J females.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | Expression of lacZ is detected in the neural tube, forebrain, somites, ventral body wall, heart, gut and skeletal muscle. |
Allele Name | gene trap ROSA53, Philippe Soriano |
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Allele Type | Gene trapped (Reporter, Null/Knockout) |
Allele Synonym(s) | shrmGtrosa53; shroom; ShroomGt; Shroom3Gt(ROSA)53Sor |
Gene Symbol and Name | Shroom3, shroom family member 3 |
Gene Synonym(s) | |
Promoter | Shroom3, shroom family member 3, mouse, laboratory |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | Expression of lacZ is detected in the neural tube, forebrain, somites, ventral body wall, heart, gut and skeletal muscle. |
Strain of Origin | 129S4/SvJaeSor |
Chromosome | 5 |
Molecular Note | Gene trap insertion which disrupted expression of one of two alternative splice forms. Insertion occurred 3' of the translation start site for the longer transcript, but 5' to the translational start site for the shorter transcript. |
Mutations Made By | Dr. Philippe Soriano, Mount Sinai School of Medicine |
This strain originated on a B6;129S background and has been backcrossed to C57BL/6J for at least ten generations. Homozygous mice are embryonic lethal.
When using the shroom mouse strain in a publication, please cite the originating article(s) and include JAX stock #003754 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wildtype for Shroom3<Gt(ROSA53)Sor> |
Frozen Mouse Embryo | B6.129S4-Shroom3<Gt(ROSA53)Sor>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Shroom3<Gt(ROSA53)Sor>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Shroom3<Gt(ROSA53)Sor>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129S4-Shroom3<Gt(ROSA53)Sor>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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