These Stat4/Stat6 knock-out mice are defective in both IL-12 mediated signaling as well as IL-4 induced responses.
The Jackson Laboratory
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Stat4 | signal transducer and activator of transcription 4 |
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Stat6 | signal transducer and activator of transcription 6 |
Mice homozygous for both the Stat4tm1Gru and Stat6tm1Gru targeted mutations are viable and fertile. Mutant mice display characteristics of both STAT4 and STAT6 deficient mice. They are defective in both IL-12 mediated signaling as well as IL-4 induced responses. They do not generate functional Th2 cells, a characteristic of STAT6 deficient mice. However, while STAT4 deficient mice do not produce interferon gamma secreting Th1 cells, the STAT4/STAT6 double deficient mice do exhibit a functional Th1 response, producing significant amounts of IFN-{\f 23 g}. The presence of a functional Th1 response in double deficient mice indicates that there is a STAT4 independent pathway for the production of Th1 cells when STAT6 signaling is not activated.
Allele Name | targeted mutation 1, Michael J Grusby |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Stat4- |
Gene Symbol and Name | Stat4, signal transducer and activator of transcription 4 |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 1 |
Molecular Note | The exon encoding amino acids 263 through 314 was disrupted by the insertion of a neomycin selection cassette. The encoded protein was undetectable in the thymi and lymph nodes of homozygous mutant mice by immunoblot analysis. |
Mutations Made By | Dr. Michael Grusby, Harvard Medical School |
Allele Name | targeted mutation 1, Michael J Grusby |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | S6KO; Stat6- |
Gene Symbol and Name | Stat6, signal transducer and activator of transcription 6 |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 10 |
Molecular Note | The region encoding the SH2 domain, which is essential for dimerization, was replaced by the insertion of a neomycin selection cassette. Immunoblot analysis of lysates from the spleens and thymi of homozygous mutant mice showed an absence of encoded protein. |
Mutations Made By | Dr. Michael Grusby, Harvard Medical School |
Mice homozygous for both the Stat4tm1Gru and Stat6tm1Gru targeted mutations are viable and fertile. When maintaining a live colony, mice homozygous for both targeted mutations may be bred together. The expected coat color from breeding is albino.
When using the Stat4/Stat6 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #003750 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Homozygous for Stat4<tm1Gru>, Homozygous for Stat6<tm1Gru> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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