C.129S2-Stat4^tm1Gru Stat6^tm1Gru/J

Stock number: 003750
Product name: Stat4/Stat6 KO
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

These Stat4/Stat6 knock-out mice are defective in both IL-12 mediated signaling as well as IL-4 induced responses.

Detailed description

Mice homozygous for both the Stat4^tm1Gru and Stat6^tm1Gru targeted mutations are viable and fertile. Mutant mice display characteristics of both STAT4 and STAT6 deficient mice. They are defective in both IL-12 mediated signaling as well as IL-4 induced responses. They do not generate functional Th2 cells, a characteristic of STAT6 deficient mice. However, while STAT4 deficient mice do not produce interferon gamma secreting Th1 cells, the STAT4/STAT6 double deficient mice do exhibit a functional Th1 response, producing significant amounts of IFN-{\f 23 g}. The presence of a functional Th1 response in double deficient mice indicates that there is a STAT4 independent pathway for the production of Th1 cells when STAT6 signaling is not activated.

Allele

Symbol: Stat6^tm1Gru
Name: targeted mutation 1, Michael J Grusby
MGI accession ID: MGI:1888385
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Stat6
Marker name: signal transducer and activator of transcription 6
Chromosome: 10
Strain of origin: 129S2/SvPas
Molecular note: The region encoding the SH2 domain, which is essential for dimerization, was replaced by the insertion of a neomycin selection cassette. Immunoblot analysis of lysates from the spleens and thymi of homozygous mutant mice showed an absence of encoded protein.

Allele

Symbol: Stat4^tm1Gru
Name: targeted mutation 1, Michael J Grusby
MGI accession ID: MGI:1857248
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Stat4
Marker name: signal transducer and activator of transcription 4
Chromosome: 1
Strain of origin: 129S2/SvPas
Molecular note: The exon encoding amino acids 263 through 314 was disrupted by the insertion of a neomycin selection cassette. The encoded protein was undetectable in the thymi and lymph nodes of homozygous mutant mice by immunoblot analysis.