B6;129S5-Pak2^{Gt(OST104058)Lex}/J

Stock number: 003749

Detailed description

Homozygotes are embryonic lethal. The Lexicon Genetics Omnibank Sequence Tag (OST) number associated with this strain is OST104058. More sequence information is available at their website (http://www.lexgen.com/).

Importation of this model was supported by the Merck Genome Research Institute (MGRI).The MGRI strains have not been characterized in any great detail. In some instances, rudimentary expression information is available. We plan to update strain information as published results become available.

Development

The Cpsf3 gene was disrupted in Lex-1 embryonic stem cells (derived from 129S5/SvEvBrd). Selected cells were injected into C57BL/6-Tyr^c-Brd/c-Brd blastocysts.

Allele

Symbol: Pak2^{Gt(OST104058)Lex}
Name: gene trap OST104058, Lexicon Genetics
MGI accession ID: MGI:1861800
Generation method: Gene trapped
Attributes: Null/Knockout
Marker symbol: Pak2
Marker name: p21 (RAC1) activated kinase 2
Chromosome: 16
Strain of origin: 129S5/SvEvBrd
Molecular note: The gene trap cassette consisting of an long terminal repeat (LTR), splice acceptor sequence (SA), internal ribosome entry site-galactosidase/neomycin phosphotransferase fusion gene (IRES-geo), polyadenylation sequence, phosphoglycerate kinase-1 promoter (PGK), puromycin N-acetyltransferase gene, splice donor sequence (SD), and LTR, inserted before exon 2. Western blot analysis indicates that levels of protein in the hippocampus and cortex are reduced in heterozygotes.