Homozygotes are embryonic lethal. The Lexicon Genetics Omnibank Sequence Tag (OST) number associated with this strain is OST104058. More sequence information is available at their website (http://www.lexgen.com/).
Importation of this model was supported by the Merck Genome Research Institute (MGRI).The MGRI strains have not been characterized in any great detail. In some instances, rudimentary expression information is available. We plan to update strain information as published results become available.
Ramiro Ramirez-Solls, Lexicon Genetics Incorporated
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Gene trapped (Null/Knockout) | Pak2 | p21 (RAC1) activated kinase 2 |
Homozygotes are embryonic lethal. The Lexicon Genetics Omnibank Sequence Tag (OST) number associated with this strain is OST104058. More sequence information is available at their website (http://www.lexgen.com/).
Importation of this model was supported by the Merck Genome Research Institute (MGRI).The MGRI strains have not been characterized in any great detail. In some instances, rudimentary expression information is available. We plan to update strain information as published results become available.
The Cpsf3 gene was disrupted in Lex-1 embryonic stem cells (derived from 129S5/SvEvBrd). Selected cells were injected into C57BL/6-Tyrc-Brd/c-Brd blastocysts.
Allele Name | gene trap OST104058, Lexicon Genetics |
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Allele Type | Gene trapped (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Pak2, p21 (RAC1) activated kinase 2 |
Gene Synonym(s) | |
Strain of Origin | 129S5/SvEvBrd |
Chromosome | 16 |
Molecular Note | The gene trap cassette consisting of an long terminal repeat (LTR), splice acceptor sequence (SA), internal ribosome entry site-galactosidase/neomycin phosphotransferase fusion gene (IRES-geo), polyadenylation sequence, phosphoglycerate kinase-1 promoter (PGK), puromycin N-acetyltransferase gene, splice donor sequence (SD), and LTR, inserted before exon 2. Western blot analysis indicates that levels of protein in the hippocampus and cortex are reduced in heterozygotes. |
Mutations Made By | Ramiro Ramirez-Solls, Lexicon Genetics Incorporated |
Homozygotes are embryonic lethal. The expected coat colors are black, albino, and agouti.
When using the B6;129S5-Pak2Gt(OST104058)Lex/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #003749 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wildtype for Pak2<Gt(OST104058)Lex> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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