MEV-O.CAST-A D2Mit57/TyJ

Stock number: 003733

Development

MEV/1Ty was developed by crossing C58/J with AKXD-14/Ty, and generating multiple recombinant inbred lines. At F6 these lines were tested and one that was found to carry all of the multiple ecotropic MuLV proviruses of interest was expanded. At F9 one line was found to be homozygous for all but 3 of the multiple ecotropic MuLV proviruses of interest. This line was further inbred and at F12 was homozygous for the multiple ecotropic MuLV proviruses of interest along with the nonagouti and dilute coat color alleles. Proviral re-insertions were identified and characterized on MEV/1Ty and enhanced substrains of MEV/1Ty were developed that carry these proviruses. MEV-O/Ty is homozygous for Emv29, Emv47, and R34 (not assigned an Emv number). The Agouti and D2Mit57 bearing segment of chromosome 2 were backcrossed from CAST/Ei onto MEV-O/Ty via 10 generations of backcrossing and then the strain was sibling mated to homozygosity for this segment and was maintained by sibling breeding thereafter. (Taylor and Rowe, 1989, 1993, 1997.)

Allele

MGI accession ID: MGI:92193
Marker symbol: D2Mit57
Marker name: DNA segment, Chr 2, Massachusetts Institute of Technology 57
Chromosome: 2