These MT-TGF-α mice express a transgene containing DNA of the wild-type allele of the human TGFA (transforming growth factor alpha) gene, and a human growth hormone polyadenylation signal. They exhibit progressive liver lesions developing into neoplasia.
IMR Colony, The Jackson Laboratory
In the STOCK TgN(MtTGFA)42Lmb line, liver lesions develop in a progressive fashion, with neoplasia confirmed at autopsy (average age 13 months). Pancreas showed progressive hyperplasia of the stroma, tubular structures and ductular metaplasia, with penetrance of 100%. Unlike mice from the FVB/N-TgN(MtTGFA)100Lmb line, STOCK TgN(MtTGFA)42Lmb mice do not develop mammary tumors.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
|Expressed Gene||TGFA, transforming growth factor alpha, human|
|Site of Expression|
|Allele Name||transgene insertion 42, Glenn Merlino|
|Allele Type||Transgenic (Humanized sequence, Inserted expressed sequence)|
|Allele Synonym(s)||MT-TGF-alpha; MT/TGF-alpha line MT42; MT42; TGF-alpha; Tg(Mt1-TGFA)42Lmb; line MT42|
|Gene Symbol and Name||Tg(MtTGFA)42Lmb, transgene insertion 42, Glenn Merlino|
|Gene Synonym(s)||MT-TGF-alpha; MT/TGF-alpha line MT42; MT42; TGF-alpha; Tg(Mt1-TGFA)42Lmb; line MT42|
|Promoter||Mt1, metallothionein 1, mouse, laboratory|
|Expressed Gene||TGFA, transforming growth factor alpha, human|
|Strain of Origin||CD-1|
|Molecular Note||This transgene contains the inducible mouse Mt1 (metallothionein) promoter, cDNA of the wild-type allele of the human TGFA (transforming growth factor alpha) gene, and a human growth hormone polyadenylation signal. Line 100, carrying 10 copies of the transgene was also produced (see Tg(MtTGFA)100Lmb).|
|Mutations Made By|| |
Dr. Glenn Merlino, National Institutes of Health
When maintaining a live colony, hemizygous mice may be bred together, to wildtype (non-carrier) mice from the colony or to SWR/J inbred mice (Stock No. 000689).
When using the MT-TGF-α mouse strain in a publication, please cite the originating article(s) and include JAX stock #003722 in your Materials and Methods section.
|Hemizygous or Non carrier for Tg(MtTGFA)42Lmb|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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