FVB-Tg(GadGFP)45704Swn/J

Stock number: 003718
Product name: GIN
Research areas: Neurobiology Research, Research Tools

Description

Mice homozygous for Tg(GadGFP)45704Swn express Enhanced Green Fluorescent Protein (EGFP) under the control of the mouse Gad1 (GAD67) gene promoter. Transgene expression occurs in a specific subpopulation of hippocampal and cortical GABAergic interneurons that express somatostatin. This subset of interneurons has been shown to be prone to injury during epilepsy, ischemia, and Alzheimer's disease, and are useful for the morphological identification and study of these interneurons in both living and fixed brain tissue.

Detailed description

Mice homozygous for the Tg(GadGFP)45704Swn transgene express Enhanced Green Fluorescent Protein (EGFP) under the control of the mouse Gad1 (GAD67) gene promoter. Homozygous mice exhibit no apparent physical or behavioral defects. Transgene expression occurs in a specific subpopulation of hippocampal and cortical GABAergic interneurons that express somatostatin. This subset of interneurons has been shown to be prone to injury during epilepsy, ischemia, and Alzheimer's disease. These transgenic mice are useful for the morphological identification and study of these interneurons in both living and fixed brain tissue.

Of note, this strain is one of many fluorescent GABAergic neuron strains, each with unique labeling characteristics (see Stock No. 006334 and Stock No. 006340).

Development

A transgenic construct containing a 5' portion of the mouse Gad1 gene, a GFP open reading frame, and SV40 polyadenylation site sequence was injected into fertilized FVB/N mouse eggs. Founder animals were bred to wildtype FVB/NHsd mice. Homozygous mice were generated and interbred.

Allele

Symbol: Tg(GadGFP)45704Swn
Name: transgene insertion 45704, John W Swann
MGI accession ID: MGI:3505731
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(GadGFP)45704Swn
Marker name: transgene insertion 45704, John W Swann
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: Transgene expression occurs in a specific subpopulation of hippocampal and cortical GABAergic interneurons that express somatostatin.
Molecular note: The transgene contains a 5' portion of the mouse Gad1 gene, a GFP open reading frame, and SV40 polyadenylation site sequence. Homozygous transgenic mice express Enhanced Green Fluorescent Protein (EGFP) under the control of the mouse Gad1 gene promoter.
General note: Homozygous transgenic mice exhibit no apparent physical or behavioral defects. Transgene expression occurs in a specific subpopulation of hippocampal and cortical GABAergic interneurons that express somatostatin.