PWK was derived from wild mice trapped near Prague, Czech Republic. PWK has a biochemical profile that is similar to the inbred strain PWD (also originating from the Czech Republic - Stock No. 004660) with the exception of the isoenzyme variant esterase 13 (Es13b). Wild-derived mice are genetically distinct from common laboratory mice for a number of complex phenotypic characteristics and are valuable tools for genetic mapping, evolution and systematics research.
In 2019-2020, researchers at The Jackson Laboratory discovered this inbred strain contains the Trem2S148E allele - a naturally occurring variant at position 48351151-48351152 on Chr 17 (rs108080490 and rs107649577; Ensembl GRCm38.p6). This TC to GA transition results in a serine to glutamic acid substitution at amino acid 148 (S148E).
PWK/Ph is descended by sib mating from a single pair of mice of the subspecies Mus musculus musculus caught in 1974 in Lhotka, Czech Republic (Von Deimling et al. 1988). Founders were selected for their ability to produce sterile sons when mated to C57BL/10 mice. The strain was imported to The Jackson Laboratory in May 2000 from Dr Jiri Forejt, Institute of Molecular Genetics, Prague.
Allele Name | long |
---|---|
Allele Type | Not Applicable (Not Specified) |
Allele Synonym(s) | SCAF1113 |
Gene Symbol and Name | Cox7a2l, cytochrome c oxidase subunit 7A2 like |
Gene Synonym(s) | |
Strain of Origin | multiple strains |
Chromosome | 17 |
General Note | Querying the sequences of the Sanger Mouse Genomes Project reveals that the short allele with its 6 bp deletion exists in C57BL/6J, C57BL/10J, C57BL/6NJ, C58/J, BALB/cJ, C3H/HeH, 129S5/SvEvBrd, NZW/LacZ, and SEA/GnJ, but the long allele lacking the deletion exists in 129S1/SvImJ, A/J, AKR/J, BTBR T+ Itpr3tf/J, BUB/BnJ, C3H/HeJ, C57BR/cdJ, C57L/J, CAST/EiJ, CBA/J, DBA/1J, DBA/2J, FVB/NJ, I/LnJ, KK/HiJ, LEWES/EiJ, LP/J, MOLF/EiJ, NOD/ShiLtJ, NZB/BlNJ, NZO/HlLtJ, PWK/PhJ, RF/J, SPRET/EiJ, ST/bJ, WSB/EiJ, ZALENDE/EiJ. |
Molecular Note | This allele encodes the long isoform with 113 amino acids. It is found in 129S2/SvPasCrl, CBA/CaOlaHsd, Hsd:ICR, and NZB/OlaHsd. |
Allele Name | PWK/PhJ |
---|---|
Allele Type | Not Applicable (Hypomorph) |
Allele Synonym(s) | |
Gene Symbol and Name | Muc4, mucin 4 |
Gene Synonym(s) | |
Strain of Origin | PWK/PhJ |
Chromosome | 16 |
Molecular Note | Several non-synonymous coding variants exist between this allele and Muc4 in other strains. The causative mutation has yet to be identified. Reduced transcript expression was confirmed. |
When using the PWK/PhJ mouse strain in a publication, please include JAX stock #003715 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Inbred, 1 pair minimum will be supplied |
Frozen Mouse Embryo | PWK/PhJ | $2595.00 |
Frozen Mouse Embryo | PWK/PhJ | $2595.00 |
Frozen Mouse Embryo | PWK/PhJ | $3373.50 |
Frozen Mouse Embryo | PWK/PhJ | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.