PWK/PhJ

Stock number: 003715
Research areas: Reproductive Biology Research, Research Tools

Description

This wild-derived inbred strain may be useful for genetic mapping studies.

Detailed description

PWK was derived from wild mice trapped near Prague, Czech Republic. PWK has a biochemical profile that is similar to the inbred strain PWD (also originating from the Czech Republic - Stock No. 004660) with the exception of the isoenzyme variant esterase 13 (Es13^b). Wild-derived mice are genetically distinct from common laboratory mice for a number of complex phenotypic characteristics and are valuable tools for genetic mapping, evolution and systematics research.

In 2019-2020, researchers at The Jackson Laboratory discovered this inbred strain contains the Trem2^S148E allele - a naturally occurring variant at position 48351151-48351152 on Chr 17 (rs108080490 and rs107649577; Ensembl GRCm38.p6). This TC to GA transition results in a serine to glutamic acid substitution at amino acid 148 (S148E).

Development

PWK/Ph is descended by sib mating from a single pair of mice of the subspecies Mus musculus musculus caught in 1974 in Lhotka, Czech Republic (Von Deimling et al. 1988). Founders were selected for their ability to produce sterile sons when mated to C57BL/10 mice. The strain was imported to The Jackson Laboratory in May 2000 from Dr Jiri Forejt, Institute of Molecular Genetics, Prague.

Allele

Symbol: Cox7a2l^l
Name: long
MGI accession ID: MGI:6276121
Generation method: Not Applicable
Attributes: Not Specified
Marker symbol: Cox7a2l
Marker name: cytochrome c oxidase subunit 7A2 like
Chromosome: 17
Strain of origin: multiple strains
Molecular note: This allele encodes the long isoform with 113 amino acids. It is found in 129S2/SvPasCrl, CBA/CaOlaHsd, Hsd:ICR, and NZB/OlaHsd.
General note: Querying the sequences of the Sanger Mouse Genomes Project reveals that the short allele with its 6 bp deletion exists in C57BL/6J, C57BL/10J, C57BL/6NJ, C58/J, BALB/cJ, C3H/HeH, 129S5/SvEvBrd, NZW/LacZ, and SEA/GnJ, but the long allele lacking the deletion exists in 129S1/SvImJ, A/J, AKR/J, BTBR T⁺ Itpr3^tf/J, BUB/BnJ, C3H/HeJ, C57BR/cdJ, C57L/J, CAST/EiJ, CBA/J, DBA/1J, DBA/2J, FVB/NJ, I/LnJ, KK/HiJ, LEWES/EiJ, LP/J, MOLF/EiJ, NOD/ShiLtJ, NZB/BlNJ, NZO/HlLtJ, PWK/PhJ, RF/J, SPRET/EiJ, ST/bJ, WSB/EiJ, ZALENDE/EiJ.

Allele

Symbol: Muc4^PWK/PhJ
Name: PWK/PhJ
MGI accession ID: MGI:6423091
Generation method: Not Applicable
Attributes: Hypomorph
Marker symbol: Muc4
Marker name: mucin 4
Chromosome: 16
Strain of origin: PWK/PhJ
Molecular note: Several non-synonymous coding variants exist between this allele and Muc4 in other strains. The causative mutation has yet to be identified. Reduced transcript expression was confirmed.