NONcNZO3/Lt

Stock number: 003670
Product name: RCS-3

Detailed description

This is one of a series of lines of interval-directed recombinant congenic strains (RCS) with NON/Lt as the background strain. The lines were created by combining variable numbers of known quantitative trait loci (QTL) for obesity and diabetes contributed by New Zealand Obese (NZO/HlLt) and Nonobese Nondiabetic (NON/Lt) strains. Because these lines reflect different collections of obesity and diabetes syndromes QTL, they are extremely relevant as models for the polygenic obesity/ diabesity syndromes in humans.

Development

This line was created by two backcrosses to NON/Lt of the NON/Lt x NZO/HlLt F1 outcross. At the second backcross (N3), individual mice were identified that were heterozygous for NZO or NON markers previously demonstrated in either F2 or N2 segregation analysis to be associated with the diabetes subphenotypes of elevated plasma glucose (PG), elevated or lowered plasma insulin (PI), and elevated body weight. Such mice were then selectively bred to fix the diabetogenic and nondiabetogenic alleles.