These Zp3-Cre82 mice express a transgene containing regulatory sequences from the mouse zona pellucida 3 (Zp3) gene directing expression of cre exclusively in the growing oocyte.
Dr. Barbara Knowles, Institute of Medical Biology
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
This is a transgenic line in which cre expression is controlled by the regulatory sequences from the mouse zona pellucida 3 (Zp3) gene. This promoter normally directs expression exclusively in the growing oocyte prior to the completion of the first meiotic division. This strain is useful for deleting a floxed sequence specifically in the female germ line. The Donating Investigator suggests to accomplish this, females homozygous or heterozygous for the floxed allele, as well as hemizygous for the Zp3cre allele are crossed with wild type males. Progeny will carry the deleted-floxed allele.
A transgenic construct containing sequence encoding Cre recombinase, a 6Kb sequence of the mouse Zp3 promoter and the metallothionein-1 polyadenylation site sequence was injected into C57BL/6J zygotes to produce the founder line. This line was maintained on the C57BL/6J background and cryopreservation was done from homozygous males.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre recombinase is normally expressed in the growing oocyte prior to the completion of the first meiotic division. |
Allele Name | transgene insertion 82, Barbara B Knowles |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Zp3-Cretg |
Gene Symbol and Name | Tg(Zp3-cre)82Knw, transgene insertion 82, Barbara B Knowles |
Gene Synonym(s) | |
Promoter | Zp3, zona pellucida glycoprotein 3, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre recombinase is normally expressed in the growing oocyte prior to the completion of the first meiotic division. |
Strain of Origin | C57BL/6J |
Chromosome | 2 |
Molecular Note | A construct encoding Cre recombinase under the control of a 6kb sequence of the mouse Zp3 promoter was microinjected into C57BL/6J zygotes and integrated randomly into Chromosome 2. |
When using the Zp3-cre82 mouse strain in a publication, please cite the originating article(s) and include JAX stock #003650 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Homozygous for Tg(Zp3-cre)82Knw |
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