C57BL/6J-Tg(Zp3-cre)82Knw/KnwJ

Stock number: 003650
Product name: Zp3-cre82
Research areas: Research Tools

Description

These Zp3-Cre82 mice express a transgene containing regulatory sequences from the mouse zona pellucida 3 (Zp3) gene directing expression of cre exclusively in the growing oocyte.

Detailed description

This is a transgenic line in which cre expression is controlled by the regulatory sequences from the mouse zona pellucida 3 (Zp3) gene. This promoter normally directs expression exclusively in the growing oocyte prior to the completion of the first meiotic division. This strain is useful for deleting a floxed sequence specifically in the female germ line. The Donating Investigator suggests to accomplish this, females homozygous or heterozygous for the floxed allele, as well as hemizygous for the Zp3cre allele are crossed with wild type males. Progeny will carry the deleted-floxed allele.

Development

A transgenic construct containing sequence encoding Cre recombinase, a 6Kb sequence of the mouse Zp3 promoter and the metallothionein-1 polyadenylation site sequence was injected into C57BL/6J zygotes to produce the founder line. This line was maintained on the C57BL/6J background and cryopreservation was done from homozygous males.

Allele

Symbol: Tg(Zp3-cre)82Knw
Name: transgene insertion 82, Barbara B Knowles
MGI accession ID: MGI:4888024
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Zp3-cre)82Knw
Marker name: transgene insertion 82, Barbara B Knowles
Chromosome: 2
Strain of origin: C57BL/6J
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre recombinase is normally expressed in the growing oocyte prior to the completion of the first meiotic division.
Molecular note: A construct encoding Cre recombinase under the control of a 6kb sequence of the mouse Zp3 promoter was microinjected into C57BL/6J zygotes and integrated randomly into Chromosome 2.