Mice homozygous for the Il12rb2tm1Jm targeted mutation are viable and fertile. Con A-activated splenocytes from homozygous mice bind IL-12 with both high and low affinity, but fail to produce IFN-gamma or proliferate in response to IL-12 stimulation; IL-12-mediated signal transduction is defective as measured by phosphorylation of STAT4. Splenocytes fail to show induced NK lytic activity when incubated with IL-12 and are deficient in IFN-gamma secretion when stimulated with either Con A or anti-CD3 mAb in vitro. Homozygous mice are deficient in vivo in their ability to produce IFN-gamma following endotoxin administration and to generate a type 1 cytokine response.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary fro...
The Jackson Laboratory
Genetic Background | Generation |
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001026 BALB/cByJ |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Il12rb2 | interleukin 12 receptor, beta 2 |
Mice homozygous for the Il12rb2tm1Jm targeted mutation are viable and fertile. Con A-activated splenocytes from homozygous mice bind IL-12 with both high and low affinity, but fail to produce IFN-gamma or proliferate in response to IL-12 stimulation; IL-12-mediated signal transduction is defective as measured by phosphorylation of STAT4. Splenocytes fail to show induced NK lytic activity when incubated with IL-12 and are deficient in IFN-gamma secretion when stimulated with either Con A or anti-CD3 mAb in vitro. Homozygous mice are deficient in vivo in their ability to produce IFN-gamma following endotoxin administration and to generate a type 1 cytokine response.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Allele Name | targeted mutation 1, Jeanne Magram |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | IL-12Rbeta2-; IL-12Rbeta2KO |
Gene Symbol and Name | Il12rb2, interleukin 12 receptor, beta 2 |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 6 |
Molecular Note | A neomycin resistance cassette replaced exons 2 and 3 of the gene, deleting the ATG translation initiation codon, the signal peptide, and the N-terminal immunoglobulin domain of the receptor. |
Mutations Made By | Dr. David Presky, Hoffman-La Roche, Inc. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the IL-12Rss2- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003642 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Il12rb2<tm1Jm> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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