B6.129S4-Cd80^tm1Shr/J

Stock number: 003611
Research areas: Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research

Description

Cd80 (B7-1) null mice exhibit a diminished mixed lymphocyte response. Following immunization, antigen specific IgG1, IgG2a and IgM isotypes are 25%-50% that of wild type levels. Survival of certain tissue allografts are slightly prolonged in Cd80 null mice.

Detailed description

Cd80 (B7-1) null mice are viable and fertile. They possess normal numbers of B and T lymphocytes but exhibit a diminished mixed lymphocyte response. Following immunization, antigen specific IgG1, IgG2a and IgM isotypes are 25%-50% that of wild type levels. Survival of certain tissue allografts are slightly prolonged in Cd80 null mice.

Development

A targeting vector containing a neomycin gene under the control of the mouse phosphoglycerol kinase (PGK) promoter was used to disrupt the second exon containing the Ig-V-like domain in J1 embryonic stem (ES) cells. ES cells were injected in to C57BL/6J blastocysts.

SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed that the Donating Investigator used C57BL/6N (2 of 5 markers segregating) in teh development of the strain. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J at least once to establish the colony.

Allele

Symbol: Cd80^tm1Shr
Name: targeted mutation 1, Arlene H Sharpe
MGI accession ID: MGI:1861804
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Cd80
Marker name: CD80 antigen
Chromosome: 16
Strain of origin: 129S4/SvJae
Molecular note: A neomycin selection cassette replaced the exon encoding the IgV-like domain of the protein. RT-PCR analysis on RNA derived from spleen, thymus and LPS-stimulated B cells of homozygous mice demonstrated that no detectable transcript was produced from this allele. FACS analysis on B cells derived from homozygous mice confirmed that no functional protein was encoded by this allele.