Cd80 (B7-1) null mice exhibit a diminished mixed lymphocyte response. Following immunization, antigen specific IgG1, IgG2a and IgM isotypes are 25%-50% that of wild type levels. Survival of certain tissue allografts are slightly prolonged in Cd80 null mice.
Arlene H Sharpe, Harvard Medical School
Cd80 (B7-1) null mice are viable and fertile. They possess normal numbers of B and T lymphocytes but exhibit a diminished mixed lymphocyte response. Following immunization, antigen specific IgG1, IgG2a and IgM isotypes are 25%-50% that of wild type levels. Survival of certain tissue allografts are slightly prolonged in Cd80 null mice.
A targeting vector containing a neomycin gene under the control of the mouse phosphoglycerol kinase (PGK) promoter was used to disrupt the second exon containing the Ig-V-like domain in J1 embryonic stem (ES) cells. ES cells were injected in to C57BL/6J blastocysts.
SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed that the Donating Investigator used C57BL/6N (2 of 5 markers segregating) in teh development of the strain. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J at least once to establish the colony.
|Allele Name||targeted mutation 1, Arlene H Sharpe|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||B7-; B7.1; B7-1-; CD80-|
|Gene Symbol and Name||Cd80, CD80 antigen|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A neomycin selection cassette replaced the exon encoding the IgV-like domain of the protein. RT-PCR analysis on RNA derived from spleen, thymus and LPS-stimulated B cells of homozygous mice demonstrated that no detectable transcript was produced from this allele. FACS analysis on B cells derived from homozygous mice confirmed that no functional protein was encoded by this allele.|
|Mutations Made By|| |
Arlene Sharpe, Harvard Medical School
This strain originated on a B6/129S4 background. It was backcrossed to C57BL/6 for ten generations and has been maintained by intercrosses since N10. It is maintained as a homozygote. Coat color expected from breeding:Black
When using the B6.129S4-Cd80tm1Shr/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #003611 in your Materials and Methods section.