These NOD mice contain a chromosome 7 segment from C57BL/6 delineated by the flanking markers Gpib and D7Mit346.
Dr. Edward Leiter, The Jackson Laboratory
NOD.B6-(Gpi1-D7Mit346)/LtJ mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Congenic NOD Gpib mice become diabetic at a similar rate and onset as NOD/Lt (Chen et al, 2005). Gpi1b in these mice can be used as a marker for transplant studies.
A C57BL/6 derived, Chr 7 genomic segment extending from Gpi1b to D7Mit346 was transferred to NOD/Lt (Gpi1a). An (NODxC57BL/6J)F1 was backcrossed to NOD/Lt for 11 generations with selection for C57BL/6 markers on Chr. 7 prior to inbreeding.
|Allele Name||b variant|
|Allele Type||Not Applicable|
|Gene Symbol and Name||Gpi1, glucose phosphate isomerase 1|
|Strain of Origin||Not Applicable|
|Molecular Note||This allele determines a faster migrating variant of the encoded enzyme and occurs in the wild and in strains BUB/Bn, CBA/J, CBA/CaJ, C3H/HeH, C3Heb/FeJ, C57BL/6J, C57BL/6J-ob, SEA/GJ and SWR/J. Sequencing analysis identified four nucleotide differences between this allele and Gpi1a. Two differences were silent, while a third difference at nucleotide position 282 predicts an aspartate for the a allele and an asparagine for the b allele at amino acid position 94. A fourth difference in the 3' untranslated region was also noted.|