NOD.B6-(Gpi1-D7Mit346)/LtJ

Stock number: 003585
Product name: NOD.Gpi
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These NOD mice contain a chromosome 7 segment from C57BL/6 delineated by the flanking markers Gpi^b and D7Mit346.

Detailed description

NOD.B6-(Gpi1-D7Mit346)/LtJ mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Congenic NOD Gpi^b mice become diabetic at a similar rate and onset as NOD/Lt (Chen et al, 2005). Gpi1^b in these mice can be used as a marker for transplant studies.

Development

A C57BL/6 derived, Chr 7 genomic segment extending from Gpi1^b to D7Mit346 was transferred to NOD/Lt (Gpi1^a). An (NODxC57BL/6J)F1 was backcrossed to NOD/Lt for 11 generations with selection for C57BL/6 markers on Chr. 7 prior to inbreeding.

Allele

Symbol: Gpi1^b
Name: b variant
MGI accession ID: MGI:2177270
Generation method: Not Applicable
Marker symbol: Gpi1
Marker name: glucose phosphate isomerase 1
Marker synonyms: AMF; autocrine motility factor; GNPI; Gpi-1; Gpi-1r; Gpi1-r; Gpi-1s; Gpi1-s; Gpi-1t; Gpi1-t; maturation factor; MF; neuroleukin; NK; NK/GPI; Nlk; Org; PGI; Phi; SA36; SA-36
Chromosome: 7
Strain of origin: Not Applicable
Molecular note: This allele determines a faster migrating variant of the encoded enzyme and occurs in the wild and in strains BUB/Bn, CBA/J, CBA/CaJ, C3H/HeH, C3Heb/FeJ, C57BL/6J, C57BL/6J-ob, SEA/GJ and SWR/J. Sequencing analysis identified four nucleotide differences between this allele and Gpi1^a. Two differences were silent, while a third difference at nucleotide position 282 predicts an aspartate for the a allele and an asparagine for the b allele at amino acid position 94. A fourth difference in the 3' untranslated region was also noted.