STOCK Grm4^tm1Hpn/J

Stock number: 003576
Product name: mGluR4 KO
Research areas: Neurobiology Research

Description

These Grm4 knock-out mice exhibit an impaired ability to learn complex motor tasks.

Detailed description

Metabotropic glutamate receptors are widely distributed throughout the central nervous system. Included in this group is the glutamate receptor, metabotropic 4 (also called mGluR4). The targeted gene product is found at modest levels in several areas of the CNS, but it appears to be expressed at higher levels in the granule cells of the cerebellum. No protein product is detected in the brain by immunological assays. Mutant mice appear normal in size/weight and have normal motor coordination but appear to have an impaired ability to learn complex motor tasks. The donating investigators note that these mice are selectively resistant to chemically-induced absence seizures.

Development

The targeted gene is disrupted by the insertion of a PGKneo cassette into an EcoRV site located in the putative extracellular domain. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to CD-1.

Allele

Symbol: Grm4^tm1Hpn
Name: targeted mutation 1, David R Hampson
MGI accession ID: MGI:1857980
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Grm4
Marker name: glutamate receptor, metabotropic 4
Chromosome: 17
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A neomycin resistance cassette was inserted into an EcoRV site of an exon that encodes a region encompassing Gly 246-Arg 291 of the putative amino terminal extracellular domain of the protein. Immunoblots of cerebella from homozygous mutant mice showed no detectable protein.