Metabotropic glutamate receptors are widely distributed throughout the central nervous system. Included in this group is the glutamate receptor, metabotropic 4 (also called mGluR4). The targeted gene product is found at modest levels in several areas of the CNS, but it appears to be expressed at higher levels in the granule cells of the cerebellum. No protein product is detected in the brain by immunological assays. Mutant mice appear normal in size/weight and have normal motor coordination but appear to have an impaired ability to learn complex motor tasks. The donating investigators note that these mice are selectively resistant to chemically-induced absence seizures.
The targeted gene is disrupted by the insertion of a PGKneo cassette into an EcoRV site located in the putative extracellular domain. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to CD-1.
|Allele Name||targeted mutation 1, David R Hampson|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Gprc1dtm1Hpn; mGluR4-|
|Gene Symbol and Name||Grm4, glutamate receptor, metabotropic 4|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A neomycin resistance cassette was inserted into an EcoRV site of an exon that encodes a region encompassing Gly 246-Arg 291 of the putative amino terminal extracellular domain of the protein. Immunoblots of cerebella from homozygous mutant mice showed no detectable protein.|
|Mutations Made By|| |
David Hampson, University of Toronto
When maintaining a live colony, these mice are bred by homozygote sibling matings.
When using the mGluR4 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #003576 in your Materials and Methods section.