B6.Cg-Tg(LCK-NFKBIA)5Dwb/J

Stock number: 003567
Product name: IκBα(ΔN)
Research areas: Cell Biology Research, Immunology, Inflammation and Autoimmunity Research

Description

These IκBα(ΔN) mice express a transgene containing an amino acid-truncated human NFKBIA gene fused to a FLAG epitope and exhibit a significant proliferative defect and impairment of normal T cell development.

Detailed description

Mice hemizygous for TgN(LCK-NFKBIA)5Dwb transgene exhibit a complete inactivation of the NFKB signalling pathway. The T cell lineage expresses a trans-dominant form of IkBa that constitutively represses the activity of multiple NFKB/Rel proteins. Transgenic cells expressing this inhibitor exhibit a significant proliferative defect. Mitogenic stimulation of splenocytes leads to increased apoptosis of transgenic T cells. Transgene expression also impairs the development of normal T cell populations as evidenced by diminished numbers of TCR^hiCD8 single-positive thymocytes. This defect is significantly amplified in the periphery and is accompanied by a decrease in CD4⁺ T cells.

Allele

Symbol: Tg(LCK-NFKBIA)5Dwb
Name: transgene insertion 5, Dean W Ballard
MGI accession ID: MGI:3056807
Generation method: Transgenic
Attributes: Dominant negative; Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(LCK-NFKBIA)5Dwb
Marker name: transgene insertion 5, Dean W Ballard
Chromosome: UN
Strain of origin: C57BL/6 x DBA/2
Expressed genes: NFKBIA,NFKB inhibitor alpha,human
Molecular note: An amino acid-truncated human NFKBIA gene (coding for amino acids 37-317) fused to a FLAG epitope was placed under the control of a human LCK promoter. The protein encoded by this transgene lacks sequences required for signal-dependent degradation and functions as a constitutive repressor of multiple NK-kappaB pathways. Transgene expression was readily detected in the thymus and spleen as determined by immunoblot analysis.