These TTR-GLVP mice express a transgene containing a chimeric transactivator (GLVP) driven by the transthyretin enhancer/promoter.
Dr. Sophia Y. Tsai, Baylor College of Medicine
The donating investigators report an inducible binary transactivation system that permits the control of transgene expression in a tissue-specific and inducible fashion in mice. Transcription of a target transgene is kept silent until turned on by the application of an exogenous compound. No phenotypic defects are involved. The components of the construct used includes insulator DNA segments (5' region of chicken beta globin gene) flanking both ends of the GLVP transactivator which is driven by the transthyretin enhancer/promoter. The GLVP portion consists of a mutated human progesterone receptor ligand binding domain fused to the yeast GAL4 DNA binding domain and the HSV VP16 transcriptional activation domain. In the published reference, donating investigators crossed the GLVP transactivator mouse with a second target mouse line (17x4-tk-hGH). The resulting bigenic mice harboring both transgenes, produce human growth hormone in response to RU486 (mifepristone). Analogous systems have been used for tissue specific knock out by induction of CRE recombination and to induce inhibin, rescuing an inhibin knock out mouse.
|Expressed Gene||PGR, progesterone receptor, human|
|Site of Expression|
|Allele Name||transgene insertion 1074, Sophia Y Tsai|
|Allele Type||Transgenic (Inserted expressed sequence)|
|Gene Symbol and Name||Tg(TTR-GLVP)1074Tsa, transgene insertion 1074, Sophia Y Tsai|
|Promoter||TTR, transthyretin, human|
|Expressed Gene||PGR, progesterone receptor, human|
|Strain of Origin||Not Specified|
|General Note||Transgenic mice display no phenotypic effects.|
|Molecular Note||The transgene construct includes insulator DNA segments (5' region of chicken beta globin gene) flanking both ends of the GLVP transactivator which is driven by the transthyretin enhancer/promoter. The GLVP portion consists of a mutated human progesterone receptor ligand binding domain fused to the yeast GAL4 DNA binding domain and the HSV VP16 transcriptional activation domain.|
|Mutations Made By|| |
Dr. Sophia Tsai, Baylor College of Medicine
This strain was generated and is maintained in an ICR X C57BL6 background. Authors indicate no breeding problems. Expected coat color from breeding:Albino
When using the TTR-GLVP mouse strain in a publication, please cite the originating article(s) and include JAX stock #003549 in your Materials and Methods section.
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